{"product_id":"spopl-antibody-speckle-type-poz-protein-like-bha17135803","title":"SPOPL Antibody \/ Speckle-type POZ protein-like","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPOPL Antibody \/ Speckle-type POZ protein-like is a anti-SPOPL Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPOPL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPOPL antibody detects Speckle-type POZ protein-like, a nuclear adaptor protein that regulates protein ubiquitination and transcriptional repression. Encoded by the SPOPL gene on chromosome 2q22.1, this protein is a homolog of SPOP (Speckle-type POZ protein) and functions as a substrate adaptor within the CUL3-based E3 ubiquitin ligase complex. SPOPL recruits specific nuclear proteins for ubiquitin-dependent degradation, thereby modulating chromatin structure, transcriptional activity, and protein homeostasis.\u003cbr\u003e\u003cbr\u003eStructurally, SPOPL is a 367-amino-acid nuclear protein of approximately 41 kilodaltons containing a BTB\/POZ domain responsible for CUL3 interaction and a MATH domain that recognizes substrate proteins. Unlike its paralog SPOP, which forms higher-order oligomers, SPOPL exists as a stable dimer, influencing substrate specificity and ubiquitination efficiency. SPOPL localizes to nuclear speckles and chromatin-associated regions, where it coordinates the degradation of transcriptional co-regulators and chromatin modifiers.\u003cbr\u003e\u003cbr\u003eThe SPOPL antibody is widely used in cell biology, molecular genetics, and oncology research to study ubiquitin signaling, transcriptional repression, and nuclear protein quality control. Western blot analysis detects a 41 kilodalton band corresponding to SPOPL, while immunofluorescence shows punctate nuclear staining consistent with speckle localization. This antibody enables exploration of nuclear ubiquitin pathways and their effects on gene expression and chromatin remodeling.\u003cbr\u003e\u003cbr\u003eFunctionally, SPOPL forms part of the CUL3-RBX1 ubiquitin ligase complex, targeting proteins involved in chromatin regulation and transcriptional activation. By controlling the degradation of co-regulators, SPOPL influences gene silencing, cell cycle progression, and developmental signaling. Dysregulation of SPOPL expression has been linked to cancer, where altered ubiquitin signaling contributes to genomic instability and transcriptional imbalance. The SPOPL antibody provides a reliable reagent for studying these nuclear pathways and understanding adaptor-mediated substrate selection in ubiquitin ligase systems.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPOPL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPOPL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPOPL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPOPL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPOPL+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305503085,"sku":"FY12901","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_933ca8a7-fe22-4f69-ae62-8c005a4946f3.jpg?v=1782237049","url":"https:\/\/www.ebiohippo.com\/products\/spopl-antibody-speckle-type-poz-protein-like-bha17135803","provider":"BioHippo","version":"1.0","type":"link"}