{"product_id":"spred1-antibody-sprouty-related-evh1-domain-containing-protein-1-bha17135610","title":"SPRED1 Antibody \/ Sprouty-related EVH1 domain-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPRED1 Antibody \/ Sprouty-related EVH1 domain-containing protein 1 is a anti-SPRED1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPRED1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPRED1 antibody detects Sprouty-related EVH1 domain-containing protein 1 (also known as Sprouty-related protein with EVH1 domain 1), a membrane-associated signaling inhibitor that modulates the Ras\/MAPK pathway. Encoded by the SPRED1 gene on chromosome 15q14, this protein belongs to the SPRED family, which acts as a negative regulator of growth factor signaling by inhibiting RAF activation downstream of Ras. SPRED1 contains an N-terminal Enabled\/VASP homology 1 (EVH1) domain that mediates binding to other signaling proteins, a central c-Kit binding region, and a C-terminal Sprouty-related cysteine-rich domain required for membrane localization. Through these domains, SPRED1 fine-tunes MAPK activity, ensuring controlled cellular proliferation, differentiation, and migration.\u003cbr\u003e\u003cbr\u003eSPRED1 plays critical roles during embryonic development, particularly in neural crest cell differentiation and vascular morphogenesis. Its inhibitory effect on Ras signaling prevents excessive ERK activation, which could otherwise lead to developmental abnormalities or oncogenic transformation. Mutations in SPRED1 cause Legius syndrome, an autosomal dominant disorder characterized by caf�-au-lait macules, freckling, and learning difficulties that phenotypically overlap with neurofibromatosis type 1. This highlights the gene's importance in the neurofibromin-Ras regulatory axis, as SPRED1 interacts directly with neurofibromin (NF1) to recruit it to the plasma membrane for Ras inactivation.\u003cbr\u003e\u003cbr\u003eThe SPRED1 antibody is an important reagent for signal transduction, developmental biology, and cancer research. Western blot analysis identifies a 55 kilodalton band corresponding to SPRED1, while immunofluorescence reveals punctate cytoplasmic and membrane-associated staining. Expression of SPRED1 is widespread in brain, lung, liver, and vascular endothelium, reflecting its role in growth factor signaling regulation. Loss or downregulation of SPRED1 enhances Ras\/MAPK signaling and has been associated with melanoma, hepatocellular carcinoma, and acute myeloid leukemia. Conversely, forced expression of SPRED1 suppresses ERK phosphorylation and reduces tumor cell invasiveness.\u003cbr\u003e\u003cbr\u003eAt the molecular level, SPRED1 acts as a scaffold that connects neurofibromin and Ras, stabilizing NF1-mediated GTPase activation. It also interferes with RAF recruitment to the plasma membrane, thereby preventing downstream MEK and ERK activation. These inhibitory effects are critical for maintaining normal signaling thresholds. In vascular endothelial cells, SPRED1 regulates VEGF-induced angiogenesis and vessel branching. In neurons, it contributes to axon guidance and synaptic stability by modulating localized MAPK signaling. The SPRED1 antibody is thus widely used to investigate feedback mechanisms in receptor tyrosine kinase signaling and to identify dysregulated pathways in cancer and developmental syndromes.\u003cbr\u003e\u003cbr\u003eSPRED1 expression is tightly controlled by transcriptional and post-translational mechanisms, including phosphorylation and ubiquitination that determine its stability and subcellular localization. The protein's interaction with lipid rafts allows spatially restricted inhibition of MAPK signaling near the plasma membrane.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPRED1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPRED1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPRED1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPRED1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPRED1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296524653,"sku":"FY12707","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b09af53f-ec70-4358-91e8-93ba5dd251d5.jpg?v=1782237031","url":"https:\/\/www.ebiohippo.com\/products\/spred1-antibody-sprouty-related-evh1-domain-containing-protein-1-bha17135610","provider":"BioHippo","version":"1.0","type":"link"}