{"product_id":"spred2-antibody-sprouty-related-evh1-domain-containing-protein-2-bha17136348","title":"SPRED2 Antibody \/ Sprouty-related EVH1 domain-containing protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPRED2 Antibody \/ Sprouty-related EVH1 domain-containing protein 2 is a anti-SPRED2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPRED2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPRED2 (Sprouty-related EVH1 domain-containing protein 2) is a member of the SPRED protein family, which negatively regulates receptor tyrosine kinase (RTK) signaling pathways. By interacting with components of the Ras\/MAPK cascade, SPRED2 suppresses excessive ERK activation, thereby controlling cellular processes such as proliferation, differentiation, and migration. Researchers frequently use a SPRED2 antibody to investigate signaling dynamics and the molecular mechanisms of growth factor regulation.    \u003cbr\u003e\u003cbr\u003e  SPRED2 contains an N-terminal Ena\/VASP homology 1 (EVH1) domain, a central c-Kit binding domain, and a C-terminal Sprouty-related cysteine-rich domain. These structural motifs allow SPRED2 to localize to the plasma membrane and recruit signaling proteins, where it inhibits Ras and Raf activation. Employing a SPRED2 antibody allows scientists to study how this protein maintains signaling balance across a variety of cell types.    \u003cbr\u003e\u003cbr\u003e  Altered expression of SPRED2 has been linked to human disease. Loss or reduction of SPRED2 function has been associated with enhanced MAPK pathway activity, which may contribute to oncogenesis and abnormal tissue growth. Additionally, SPRED2 is studied in the context of immune regulation and inflammation, where dysregulated signaling can lead to pathological outcomes. Using a SPRED2 antibody supports research into these processes and offers insight into therapeutic strategies aimed at modulating Ras\/MAPK signaling.    \u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPRED2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPRED2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPRED2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPRED2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPRED2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326277997,"sku":"RQ8930","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_15ceb871-ac62-4d6a-bf4d-4bd43e82b715.jpg?v=1772019449","url":"https:\/\/www.ebiohippo.com\/products\/spred2-antibody-sprouty-related-evh1-domain-containing-protein-2-bha17136348","provider":"BioHippo","version":"1.0","type":"link"}