{"product_id":"spry3-antibody-sprouty-rtk-signaling-antagonist-3-bha17136286","title":"SPRY3 Antibody \/ Sprouty RTK signaling antagonist 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPRY3 Antibody \/ Sprouty RTK signaling antagonist 3 is a anti-SPRY3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPRY3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPRY3 antibody detects Sprouty RTK signaling antagonist 3, a negative regulator of receptor tyrosine kinase (RTK) signaling encoded by the SPRY3 gene located on the X and Y chromosomes (Xp22.33\/Yp11.3). SPRY3 belongs to the sprouty family of cytoplasmic signaling modulators that fine-tune growth factor receptor signaling, particularly pathways initiated by fibroblast growth factors (FGFs), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF). It is expressed in brain, lung, placenta, and testes, where it regulates cell growth, differentiation, and angiogenesis.\u003cbr\u003e\u003cbr\u003eStructurally, SPRY3 contains a conserved cysteine-rich C-terminal domain responsible for membrane association and a variable N-terminal region that mediates interaction with adapter proteins such as GRB2 and c-CBL. It belongs to the sprouty protein family (SPRY1-4), known for modulating Ras\/MAPK signaling by interfering with RAF activation. Co-localization studies show SPRY3 associated with endosomal membranes and perinuclear regions, reflecting its regulatory role at signal transduction interfaces.\u003cbr\u003e\u003cbr\u003eFunctionally, SPRY3 acts as an inhibitor of the Ras\/MAPK pathway, suppressing excessive signaling following receptor activation. It prevents cell overproliferation and supports proper developmental patterning. In neurons, SPRY3 regulates axonal branching and synaptic plasticity, while in endothelial cells it modulates angiogenic responses to VEGF. SPRY3 also interacts with E3 ubiquitin ligases such as c-CBL, influencing receptor internalization and degradation. Known downstream pathways include ERK1\/2, PI3K-AKT, and Rho family GTPase signaling.\u003cbr\u003e\u003cbr\u003eExpression of SPRY3 is developmentally regulated, with high levels observed in fetal brain and during vascular remodeling. Dysregulation or loss of SPRY3 has been linked to tumorigenesis, impaired angiogenesis, and developmental disorders. Deletion of SPRY3 in the pseudoautosomal region may contribute to neurodevelopmental phenotypes in certain chromosomal abnormalities. Pathway associations include growth factor receptor signaling, neuronal differentiation, and vascular development. In disease models, SPRY3 acts as a tumor suppressor through attenuation of mitogenic signaling.\u003cbr\u003e\u003cbr\u003eThe SPRY3 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPRY3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPRY3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPRY3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPRY3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPRY3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047324017005,"sku":"FY13384","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_37a36016-c9af-47a2-a563-0f299dfafe66.jpg?v=1782237113","url":"https:\/\/www.ebiohippo.com\/products\/spry3-antibody-sprouty-rtk-signaling-antagonist-3-bha17136286","provider":"BioHippo","version":"1.0","type":"link"}