{"product_id":"sre-reporter-lentivirus-bhv19400024","title":"SRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe serum response element (SRE) is a promoter motif that mediates transcriptional responses to growth factor and mitogen signaling, classically studied in the c-Fos promoter. The SRE is bound by serum response factor (SRF) together with ternary complex factors such as ELK-1. Growth factor stimulation activates the MAPK\/ERK cascade, leading to ERK1\/2 (MAPK3\/MAPK1)-mediated phosphorylation of ELK-1, which together with SRF drives rapid induction of immediate-early genes. This pathway controls cell proliferation, differentiation, and survival, and its dysregulation is implicated in cancer and other proliferative disorders, making SRE-driven reporters valuable for monitoring MAPK\/ERK and growth factor signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe SRE Reporter Lentivirus places a reporter gene under the control of tandem c-Fos serum response elements, providing a sensitive readout of MAPK\/ERK and growth factor signaling through serum response factor and its cofactors, including ELK-1. Reporter options include fluorescent (GFP, RFP), luminescent (firefly and Renilla luciferase), and combined GFP\/Luc and RFP\/Luc formats, with blasticidin or puromycin selection for stable reporter cell line establishment.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring MAPK\/ERK pathway activation, studying mitogen and growth factor responses, and screening modulators of this signaling axis.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, GFP\/Luc, Luc, Renilla Luc, RFP, RFP\/Luc reporter gene under the control of tandem consensus response elements specific for the MAPK\/ERK pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251619455341,"sku":"LTV-0028-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310898962797,"sku":"LTV-0028-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310898995565,"sku":"LTV-0028-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310899028333,"sku":"LTV-0028-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310899061101,"sku":"LTV-0028-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310899093869,"sku":"LTV-0028-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310899126637,"sku":"LTV-0028-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0028-SRE-TAG-Puro-Fig.-2-FBS-treatment.jpg?v=1776934451","url":"https:\/\/www.ebiohippo.com\/products\/sre-reporter-lentivirus-bhv19400024","provider":"BioHippo","version":"1.0","type":"link"}