{"product_id":"srf-re-reporter-lentivirus-bhv19400241","title":"SRF-RE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eSerum response factor (SRF) is a transcription factor that controls genes governing cytoskeletal organization, cell proliferation, and immediate-early gene expression. SRF activity is regulated by two principal input branches: the MAPK-ELK pathway and the RhoA-actin-MRTF pathway. Signaling through G12\/G13-coupled receptors activates RhoGEFs such as p115-RhoGEF, which stimulate the small GTPase RhoA. RhoA-driven actin polymerization releases myocardin-related transcription factors to co-activate SRF, inducing genes involved in cytoskeletal remodeling, proliferation, and metastatic progression. An SRF-RE reporter built on serum response elements from the α-actin promoter selectively captures this RhoA-dependent branch of GPCR signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe SRF-RE Reporter Lentivirus is a transcription-factor reporter system designed to detect G12α\/G13α-mediated activation of RhoA signaling through serum response factor (SRF). It contains tandem repeats of consensus SRF response elements derived from the human α-actin promoter, optimized to read out RhoA-dependent transcriptional activation while minimizing the broad serum responsiveness of general SRE reporters. The construct couples these elements to a minimal TATA-box promoter and an upstream enhancer to drive a fluorescent or luminescent reporter (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase), with a constitutively expressed selection marker for stable polyclonal cell line generation. It is used to study GPCR-RhoA-SRF signaling, with readout by microscopy, flow cytometry, or luminometry. Supplied as purified lentiviral particles suitable for primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the SRF Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"SRF-RE-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251624534381,"sku":"LTV-0134-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362089918829,"sku":"LTV-0134-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362089951597,"sku":"LTV-0134-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SRF-RE-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362089984365,"sku":"LTV-0134-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362090017133,"sku":"LTV-0134-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362090049901,"sku":"LTV-0134-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362090082669,"sku":"LTV-0134-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SRF-RE-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362090115437,"sku":"LTV-0134-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SRF-RE-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362090148205,"sku":"LTV-0134-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SRF-RE-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362090180973,"sku":"LTV-0134-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SRF-RE-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362090213741,"sku":"LTV-0134-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/srf-re.jpg?v=1776934221","url":"https:\/\/www.ebiohippo.com\/products\/srf-re-reporter-lentivirus-bhv19400241","provider":"BioHippo","version":"1.0","type":"link"}