{"product_id":"ssh1-antibody-slingshot-protein-phosphatase-1-bha17136051","title":"SSH1 Antibody \/ Slingshot protein phosphatase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSSH1 Antibody \/ Slingshot protein phosphatase 1 is a anti-SSH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, cytoskeleton, nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SSH1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSSH1 antibody detects Slingshot protein phosphatase 1, a serine\/threonine phosphatase that regulates actin filament dynamics by dephosphorylating cofilin. The UniProt recommended name is Slingshot protein phosphatase 1 (SSH1). This cytoplasmic enzyme is crucial for actin cytoskeleton remodeling during cell motility, adhesion, and morphogenesis.\u003cbr\u003e\u003cbr\u003eFunctionally, SSH1 antibody identifies a 1,047-amino-acid phosphatase that activates cofilin by removing inhibitory phosphate groups at serine 3. SSH1 operates downstream of Rho family GTPases and is controlled by 14-3-3 proteins, oxidative signals, and phosphorylation. It promotes lamellipodia formation and cytoskeletal reorganization during migration and neuronal outgrowth.\u003cbr\u003e\u003cbr\u003eThe SSH1 gene is located on chromosome 12q24.13 and is expressed in brain, muscle, and epithelial tissues. SSH1 integrates signals from LIM kinase and Rac\/Cdc42 pathways to coordinate actin turnover and filament severing. Through cofilin activation, SSH1 drives rapid actin recycling necessary for directional movement and cell shape maintenance.\u003cbr\u003e\u003cbr\u003ePathologically, altered SSH1 activity contributes to cancer metastasis, neurological disorders, and cardiac hypertrophy. Dysregulated actin remodeling via SSH1-cofilin imbalance leads to defective migration and cytoskeletal instability. Research using SSH1 antibody supports studies in cytoskeletal dynamics, phosphatase signaling, and cell motility.\u003cbr\u003e\u003cbr\u003eSSH1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect actin-regulating phosphatases. \u003cbr\u003e\u003cbr\u003eStructurally, Slingshot protein phosphatase 1 contains an N-terminal cofilin-binding region and a catalytic domain related to the dual-specificity phosphatase family. This antibody aids in analyzing SSH1's control over actin filament turnover and cellular movement.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SSH1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SSH1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SSH1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SSH1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SSH1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314448749,"sku":"FY13149","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f8a3a875-9068-497e-ab21-d4b544cc251f.jpg?v=1782237101","url":"https:\/\/www.ebiohippo.com\/products\/ssh1-antibody-slingshot-protein-phosphatase-1-bha17136051","provider":"BioHippo","version":"1.0","type":"link"}