{"product_id":"ssh3-antibody-slingshot-homolog-3-bha17135981","title":"SSH3 Antibody \/ Slingshot homolog 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSSH3 Antibody \/ Slingshot homolog 3 is a anti-SSH3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nuclear speckles, cytoplasm, plasma membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SSH3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSSH3 antibody detects Slingshot homolog 3, a member of the slingshot family of serine\/threonine phosphatases that regulate actin filament dynamics through dephosphorylation of cofilin. The UniProt recommended name is Slingshot homolog 3 (SSH3). This phosphatase modulates cytoskeletal reorganization, cell migration, and synaptic remodeling by maintaining the balance between phosphorylated and active cofilin.\u003cbr\u003e\u003cbr\u003eFunctionally, SSH3 antibody identifies a 575-amino-acid cytoplasmic enzyme that acts downstream of Rho family GTPases. SSH3 dephosphorylates cofilin at serine 3, restoring its actin-severing activity and promoting filament turnover. The protein also interacts with scaffolding components such as 14-3-3 proteins and LIM kinases, coordinating actin remodeling in response to extracellular cues.\u003cbr\u003e\u003cbr\u003eThe SSH3 gene is located on chromosome 3p21.31 and is widely expressed in brain, muscle, and epithelial tissues. SSH3 contributes to cell shape changes, migration, and polarity through dynamic actin regulation. In neurons, it participates in synaptic plasticity and axon guidance by fine-tuning cytoskeletal rearrangements during development and signaling.\u003cbr\u003e\u003cbr\u003ePathologically, aberrant SSH3 activity disrupts cytoskeletal organization and has been associated with cancer metastasis and neurological disorders. Overactivation enhances cell motility and invasion, whereas loss of function impairs normal morphogenesis. Research with SSH3 antibody supports studies on actin regulation, neuronal development, and signal transduction.\u003cbr\u003e\u003cbr\u003eSSH3 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry, enabling precise detection of SSH3 in various cell types. \u003cbr\u003e\u003cbr\u003eStructurally, SSH3 contains an N-terminal catalytic domain related to the dual-specificity phosphatase family and C-terminal regions mediating protein-protein interactions. Its localization and activity are regulated by phosphorylation and 14-3-3 binding. This antibody facilitates analysis of SSH3's role in actin filament turnover and cell motility control.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SSH3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SSH3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SSH3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SSH3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SSH3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311532397,"sku":"FY13079","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5c81a3e8-bc34-4463-9129-219251d30a70.jpg?v=1782237081","url":"https:\/\/www.ebiohippo.com\/products\/ssh3-antibody-slingshot-homolog-3-bha17135981","provider":"BioHippo","version":"1.0","type":"link"}