{"product_id":"stable-hepg2-expressing-murine-adh1-va-13-cell-line-bhc10901525","title":"Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eStable HepG2 Expressing Murine ADH1 (VA-13) Cell Line is recommended for use to study the production of acetaldehyde and its effects in dysfunctions in alcoholic liver disease. The cell line expresses mouse ADH1 (Class 1 Alcohol Dehydrogenase) which oxidizes allyl alcohol to produce toxic acetaldehyde.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line is supplied as an engineered cell line derived from Human liver.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 400 µg\/ml Zeocin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in liver cancer biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe Va-13 cells may use used to investigate ethanol metabolism. Donor\/background information provided for this product: Male, 15, White, Hepatocellular carcinoma. Expression information reported for the model: Mouse ADH1 (Class 1 Alcohol Dehydrogenase).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 400 µg\/ml Zeocin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180528525677,"sku":"T3195","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DNmqR5W1Gzy9hbRkRzhyBs4voulrSAyTQnVPMAue.png?v=1774957825","url":"https:\/\/www.ebiohippo.com\/products\/stable-hepg2-expressing-murine-adh1-va-13-cell-line-bhc10901525","provider":"BioHippo","version":"1.0","type":"link"}