{"product_id":"stable-nih3t3-overexpressing-mutant-ha-r-ras2-q72h-cell-line-jlr73-bhc10922952","title":"Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 Q72H Cell Line (JLR73)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis cell line (JLR73) stably overexpresses mutant human HA-R-RAS2 Q72H protein. Additional cell lines from this panel stably overexpress various HA-R-RAS2 mutants.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 Q72H Cell Line (JLR73) is supplied as an engineered cell line derived from Mouse embryo.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, elongated star-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in embryo biology, phenotype comparison, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThese cell lines are useful models for investigating the role of HA-R-RAS2 in tumor pathology. Complete list of cell lines expressing mutant HA-R-RAS2 within this panel: T3330 - Stable NIH3T3 Overexpressing Wildtype HA-R-RAS2 Cell Line (JLR88)T3331 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 G23A Cell Line (JLR14)T3332 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 G23C Cell Line (JLR71)T3333 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 G23V Cell Line (JLR89)T3334 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 G23S Cell Line (JLR 15)T3335 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 G24C Cell Line (JLR17)T3336 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 G24D Cell Line (JLR32)T3337 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 G24V Cell Line (JLR18)T3338 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 D44E Cell Line (JLR45)T3339 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 P45R Cell Line (JLR46)T3340 - StableNIH3T3 Overexpressing Mutant HA-R-RAS2 A70T Cell Line (JLR31)T3341 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 Q72L Cell Line (JLR44)T3342 - Stable NIH3T3 Overexpressing Mutant HA-R-RAS2 Q72H Cell Line (JLR73)\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:5 to 1:10\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 36\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180547268973,"sku":"T3342","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/yUkFIofWch4XcuWvxc816y1H6HuWUIeFYfDM6XZ7.png?v=1774957873","url":"https:\/\/www.ebiohippo.com\/products\/stable-nih3t3-overexpressing-mutant-ha-r-ras2-q72h-cell-line-jlr73-bhc10922952","provider":"BioHippo","version":"1.0","type":"link"}