{"product_id":"stat4-reporter-lentivirus-bhv19400051","title":"STAT4 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eSTAT4 (Signal Transducer and Activator of Transcription 4) is a latent cytoplasmic transcription factor of the JAK\/STAT signaling pathway. In response to IL-12, STAT4 is phosphorylated by receptor-associated Janus kinases, dimerizes, and translocates to the nucleus to activate target genes. STAT4 is essential for the differentiation of naive CD4+ T cells into Th1 cells and for IFNγ production, and it contributes to IFNγ and IL-21 production in follicular helper T cells during antiviral responses. STAT4 also promotes natural killer cell antiviral activity by regulating Runx1 and Runx3. Because of its central role in cell-mediated immunity, STAT4 is an important target in immunology and inflammation research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe STAT4 Reporter Lentivirus is a transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of STAT4 activity in mammalian cells. It uses tandem repeats of STAT4 responsive elements from the human T-bet enhancer, with optimized flanking nucleotides that bias the readout toward STAT4 over the related STAT1, so the reporter responds preferentially to IL-12 signaling. A constitutively expressed selection marker and optional secondary reporter enable stable polyclonal reporter cell lines.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration gives consistent reporter expression in dividing and post-mitotic cells, including primary and thawed cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation. The system is used to study STAT4 activity and JAK\/STAT signaling, with readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the STAT4 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251625288045,"sku":"LTV-0055-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310900535661,"sku":"LTV-0055-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310900568429,"sku":"LTV-0055-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310900601197,"sku":"LTV-0055-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310900633965,"sku":"LTV-0055-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310900666733,"sku":"LTV-0055-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0055-STAT4-Reporter.jpg?v=1776934148","url":"https:\/\/www.ebiohippo.com\/products\/stat4-reporter-lentivirus-bhv19400051","provider":"BioHippo","version":"1.0","type":"link"}