{"product_id":"stat5a-b-antibody-signal-transducer-and-activator-of-transcription-5-bha17135681","title":"STAT5A\/B Antibody \/ Signal transducer and activator of transcription 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSTAT5A\/B Antibody \/ Signal transducer and activator of transcription 5 is a anti-STAT5A\/B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e STAT5A\/B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSTAT5A\/B antibody detects Signal transducer and activator of transcription 5A and 5B, two closely related transcription factors that mediate cytokine, hormone, and growth factor signaling. Encoded by the STAT5A and STAT5B genes on chromosome 17q21.2, these proteins are members of the STAT family that become phosphorylated and activated downstream of Janus kinases (JAKs). STAT5A and STAT5B share more than 90% sequence identity and function cooperatively to regulate transcription of genes involved in proliferation, survival, and differentiation, particularly in hematopoietic, immune, and mammary tissues.\u003cbr\u003e\u003cbr\u003eUpon activation by cytokines such as prolactin, interleukin-2, and growth hormone, STAT5A\/B are phosphorylated on a conserved tyrosine residue (Y694 in STAT5A, Y699 in STAT5B), leading to dimerization and nuclear translocation. In the nucleus, they bind specific DNA motifs (GAS elements) to induce transcription of target genes including BCL2L1, CISH, SOCS2, and CCND1. These targets control cell survival, feedback inhibition, and differentiation pathways across multiple systems. STAT5A plays key roles in mammary gland development and lactation, while STAT5B predominantly regulates growth hormone signaling and immune function.\u003cbr\u003e\u003cbr\u003eThe STAT5A\/B antibody is widely used in signal transduction, oncology, and immunology research to detect both STAT5 isoforms in total or phosphorylated forms. Western blot analysis identifies bands near 90 kilodaltons corresponding to STAT5A and STAT5B, while immunofluorescence reveals nuclear accumulation following cytokine stimulation. This antibody supports studies of JAK\/STAT pathway activation, cytokine signaling, and transcriptional regulation in various tissues.\u003cbr\u003e\u003cbr\u003eDysregulation of STAT5 signaling is implicated in leukemias, lymphomas, and solid tumors, where constitutive activation promotes oncogenic transformation and resistance to apoptosis. Aberrant STAT5 activity also contributes to autoimmune and inflammatory diseases. The STAT5A\/B antibody enables researchers to evaluate STAT pathway activation in both physiological and pathological contexts.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=STAT5A\/B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STAT5A\/B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=STAT5A\/B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/STAT5A\/B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STAT5A\/B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299572077,"sku":"FY12778","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2fc0c41b-1f78-4073-81e0-881933729a7c.jpg?v=1782237046","url":"https:\/\/www.ebiohippo.com\/products\/stat5a-b-antibody-signal-transducer-and-activator-of-transcription-5-bha17135681","provider":"BioHippo","version":"1.0","type":"link"}