{"product_id":"staurosporine-bhb21300333","title":"Staurosporine","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e [9S-(9a,10b,11b,13a)]-2,3,10,11,12,13-Hexahydro-10- meth oxy-9-methyl-11-(methylamino)-9,13-epoxy-1H,9H-diindolo [1,2,3- gh:3',2',1'-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Antibiotic AM-2282\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 62996-74-1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C28H26N4O3.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;98%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 10 nM - 1 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PKA, PKC, PKG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Natural\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e Staurosporine potently inhibits a variety of kinases including PKC (IC50 = 0.7 nM), PKA (IC50 = 7 nM) and PKG (IC50 = 8.5 nM)1,2. It also inhibits CaMK (IC50 = 20 nM)3 and MLCK (IC50 = 1.3 nM)1,2. In addition, Staurosporine can act as an agonist promoting neurite outgrowth4. At higher concentrations, Staurosporine induces apoptosis5.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e Antibiotic AM-2282\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eStaurosporine is a member of the K252a family of fungal alkaloids produce by Streptomyces staurospores.1,2 It is one of the most potent, cell permeable protein kinase inhibitors, and it is often used to study the involvement of protein kinases in signal transduction pathways (IC50 0.7-20 nM).3-10In pheochromocytoma PC12 cells, neuroblastoma and brain primary culture Staurosporine is a functional neurotropins agonist promoting neurite outgrowth (EC50 = 50 nM).11-12 At concentrations higher then 0.5 mM, Staurosporine generates apoptosis in several different cell types or induces G1 and G2 phase arrest in normal cells.13-16\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"0.1 mg \/ 1","offer_id":53249714422125,"sku":"S-350-0P1MG-1","price":107.0,"currency_code":"USD","in_stock":true},{"title":"0.47 mg \/ 1","offer_id":53311577588077,"sku":"S-350-0P47MG-1","price":448.0,"currency_code":"USD","in_stock":true},{"title":"1 mg \/ 1","offer_id":53311577620845,"sku":"S-350-1MG-1","price":614.0,"currency_code":"USD","in_stock":true},{"title":"50 mcg \/ 1","offer_id":53311577653613,"sku":"S-350-50MCG-1","price":75.0,"currency_code":"USD","in_stock":true},{"title":"50 mcg \/ 5","offer_id":53311577686381,"sku":"S-350-50MCG-5","price":282.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/S-350_b.gif?v=1778544456","url":"https:\/\/www.ebiohippo.com\/products\/staurosporine-bhb21300333","provider":"BioHippo","version":"1.0","type":"link"}