{"product_id":"stim2-antibody-stromal-interaction-molecule-2-bha17136288","title":"STIM2 Antibody \/ Stromal interaction molecule 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSTIM2 Antibody \/ Stromal interaction molecule 2 is a anti-STIM2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Rat. Reported localization: Cytoplasm (ER).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e STIM2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, IF, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSTIM2 antibody detects Stromal interaction molecule 2, a calcium sensor encoded by the STIM2 gene located on chromosome 4p15.2. STIM2 is an endoplasmic reticulum (ER) transmembrane protein that regulates store-operated calcium entry (SOCE) by sensing ER luminal calcium levels and activating plasma membrane Orai calcium channels. It belongs to the STIM family along with STIM1 but plays a more subtle role in maintaining basal calcium levels and preventing cytosolic calcium depletion. STIM2 is expressed widely, with high levels in brain, skeletal muscle, and immune cells, where calcium signaling is crucial for cellular function.\u003cbr\u003e\u003cbr\u003eStructurally, STIM2 consists of an N-terminal luminal EF-hand domain that binds calcium, a sterile alpha motif (SAM) that senses ER calcium depletion, a single transmembrane region, and a cytoplasmic coiled-coil domain that interacts with Orai and TRPC channels. It belongs to the stromal interaction molecule family of calcium sensors. Co-localization studies show STIM2 distributed in the ER membrane, clustering near plasma membrane regions during calcium signaling activation.\u003cbr\u003e\u003cbr\u003eFunctionally, STIM2 modulates calcium entry by maintaining the readiness of Orai1 channels for activation. It operates at lower calcium threshold levels than STIM1, allowing fine-tuned regulation of basal calcium homeostasis. In neurons, STIM2 regulates synaptic activity and plasticity by modulating calcium-dependent gene transcription. In T lymphocytes, it supports sustained calcium signaling required for cytokine production. Known interaction partners include Orai1, TRPC1, and STIM1, forming calcium entry complexes at ER-plasma membrane junctions.\u003cbr\u003e\u003cbr\u003eSTIM2 plays a critical role in various physiological processes including muscle contraction, neuronal signaling, and immune cell activation. Dysregulation of STIM2 has been linked to neurodegenerative diseases, immunodeficiency, and cardiac hypertrophy. Reduced STIM2 expression impairs calcium-dependent transcription, while overexpression disrupts calcium oscillations. Pathway associations include calcium signaling, ER stress response, and MAPK pathway activation. During development, STIM2 expression supports neuronal differentiation and synaptic maturation.\u003cbr\u003e\u003cbr\u003eThe STIM2 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=STIM2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STIM2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=STIM2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/STIM2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STIM2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047324606829,"sku":"FY13386","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7cd983ca-0187-482d-b2b2-c8ef3e048361.jpg?v=1782237114","url":"https:\/\/www.ebiohippo.com\/products\/stim2-antibody-stromal-interaction-molecule-2-bha17136288","provider":"BioHippo","version":"1.0","type":"link"}