{"product_id":"sting-isre-reporter-lentivirus-bhv19400257","title":"STING\/ISRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eSTING (stimulator of interferon genes, encoded by TMEM173) is a central adaptor of the cGAS-STING innate immune pathway. Cyclic GMP-AMP synthase (cGAS) senses cytosolic double-stranded DNA from pathogens or damaged cells and produces the cyclic dinucleotide cGAMP, which activates STING. STING then drives TBK1-dependent phosphorylation of IRF3 and activation of NF-κB, inducing type I interferons and inflammatory cytokines. Interferon-stimulated response elements (ISRE) integrate this signaling output. The cGAS-STING axis is essential for antiviral defense and antitumor immunity, and STING agonists are actively investigated as immunotherapies that reshape the tumor microenvironment.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe STING\/ISRE Reporter Lentivirus is a two-vial system for evaluating activation of the cGAS-STING pathway. Vial 1 constitutively expresses STING with an antibiotic selection marker, while Vial 2 carries tandem ISRE elements driving a dual reporter combining secreted Gaussia luciferase (GLuc) and a fluorescent protein (GFP or RFP). Sequential transduction and selection generate a stable, sensitive reporter cell line for quantifying interferon-pathway induction. Secreted GLuc enables kinetic sampling from conditioned media without cell lysis, while fluorescence supports microscopy and flow cytometry. The system is used to screen cGAS-STING agonists and study their effects on innate immune activation, antigen-presenting cells, and antitumor responses. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human STING Assay under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"STING(R232)-GFP\/ISRE-RFP \/ RFP \/ 2x10^6","offer_id":53251625222509,"sku":"TRV-0008-1S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"STING(R232)-RFP\/ISRE-GFP \/ GFP \/ 2x10^6","offer_id":53362085069165,"sku":"TRV-0008-2S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"STING(R232)-BSD\/ISRE-GFP \/ GFP \/ 2x10^6","offer_id":53362085101933,"sku":"TRV-0008-3S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"STING(R232)-BSD\/ISRE-RFP \/ RFP \/ 2x10^6","offer_id":53362085134701,"sku":"TRV-0008-4S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"STING(R232)-BSD\/ISRE-GFP-GLuc \/ GFP-P2A-GLuc \/ 2x10^6","offer_id":53362085167469,"sku":"TRV-0008-6S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"STING(H232)-BSD\/ISRE-GFP-GLuc (Low Activity) \/ GFP-P2A-GLuc \/ 2x10^6","offer_id":53362085200237,"sku":"TRV-0008-6N","price":895.0,"currency_code":"USD","in_stock":true},{"title":"Sting-GFP\/ISRE-RFP \/ RFP \/ 2x10^6","offer_id":53362085233005,"sku":"TRV-0008-1M","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261.jpg?v=1779473820","url":"https:\/\/www.ebiohippo.com\/products\/sting-isre-reporter-lentivirus-bhv19400257","provider":"BioHippo","version":"1.0","type":"link"}