{"product_id":"stk15-antibody-serine-threonine-protein-kinase-15-aurka-bha17128839","title":"STK15 Antibody \/ Serine\/threonine-protein kinase 15 \/ AURKA","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eSTK15 Antibody \/ Serine\/threonine-protein kinase 15 \/ AURKA is an antibody targeting \u003cstrong\u003eSTK15\u003c\/strong\u003e, raised in \u003cstrong\u003eRabbit\u003c\/strong\u003e for protein detection and localization studies where these specifications are required.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e STK15 (reported localization: Cytoplasmic, nuclear).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); Rabbit IgG.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Unconjugated (affects detection chemistry and multiplex compatibility).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eListed applications:\u003c\/strong\u003e WB, IHC-P, FACS, Direct ELISA (refer to on-page specifications for application-specific guidance).\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eAURKA (Aurora kinase A), also called ARK1, AurA, AIK , AURORA2, BTAK, PPP1R47, STK7, STK15, STK6, is a mitotic centrosomal protein kinase. The main role of STK15 in tumor development is in controlling chromosome segregation during mitosis. It is a member of a family of mitotic serine\/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of STK15 occurs during the G2\/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A \/ Serine\/threonine-protein kinase 15 in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of STK15 at centrosomes in late G2 phase and inhibited mitotic entry. Activation of STK15 was independently sufficient to induce rapid ciliary resorption, and STK15 acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of STK15 and HDAC6 reduced regulated disassembly of cilia.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eComparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).\u003c\/li\u003e \u003cli\u003eSubcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.\u003c\/li\u003e \u003cli\u003eIntegration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eImmunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eFlow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands\/signals accordingly.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eEpitope context:\u003c\/strong\u003e binding can depend on protein conformation and sample processing; region information in the title\/immunogen can help anticipate what may be detected.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies differences:\u003c\/strong\u003e predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include negative controls (no-primary\/isotype), and where possible genetic controls (KO\/KD) or independent antibodies to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry O14965 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/O14965 - Gene search: STK15 — NCBI Gene — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STK15 - Ensembl search: STK15 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=STK15 - PubMed search: STK15 antibody — PubMed — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STK15+antibody - Reactome search: STK15 — Reactome — https:\/\/reactome.org\/content\/query?q=STK15 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53046434988397,"sku":"RQ7272","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fa567cda-29fb-4b36-9507-a4072ba68aeb.jpg?v=1772000615","url":"https:\/\/www.ebiohippo.com\/products\/stk15-antibody-serine-threonine-protein-kinase-15-aurka-bha17128839","provider":"BioHippo","version":"1.0","type":"link"}