{"product_id":"stk33-antibody-serine-threonine-protein-kinase-33-bha17135324","title":"STK33 Antibody \/ Serine\/threonine-protein kinase 33","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSTK33 Antibody \/ Serine\/threonine-protein kinase 33 is a anti-STK33 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e STK33\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe STK33 antibody targets Serine\/threonine-protein kinase 33, a cytoplasmic kinase encoded by the STK33 gene. Serine\/threonine-protein kinase 33 phosphorylates cytoskeletal and signaling proteins to regulate apoptosis, spermatogenesis, and metabolic processes. Although its precise substrates remain under investigation, STK33 is involved in kinase cascades that control cell survival and stress adaptation. The STK33 antibody enables researchers to explore kinase signaling, protein phosphorylation, and pathways relevant to oncogenesis and germ-cell differentiation.\u003cbr\u003e\u003cbr\u003eSerine\/threonine-protein kinase 33 belongs to the calcium\/calmodulin-dependent kinase superfamily and contains a conserved catalytic domain typical of serine\/threonine kinases. It localizes mainly in the cytoplasm but may associate with actin filaments and mitochondria under stress conditions. The STK33 antibody allows localization analysis to assess how cellular context affects its distribution and activity. In germ cells, STK33 plays a role in sperm flagellum assembly and maturation, supporting reproductive function.\u003cbr\u003e\u003cbr\u003eAberrant activation of Serine\/threonine-protein kinase 33 has been associated with tumor progression in various cancers, including leukemia, glioma, and pancreatic carcinoma. The STK33 antibody supports research into these roles by enabling detection of protein levels in tumor tissues and evaluating the impact of genetic silencing or kinase inhibition. Some studies have suggested STK33 as a synthetic lethal partner with KRAS mutations, linking it to targeted cancer therapy exploration.\u003cbr\u003e\u003cbr\u003eBeyond oncology, Serine\/threonine-protein kinase 33 contributes to cytoskeletal organization and stress responses. It may phosphorylate structural proteins that stabilize actin networks and maintain cellular morphology. The STK33 antibody enables analysis of these cytoskeletal effects and supports biochemical assays aimed at identifying STK33 substrates and binding partners. Its expression has also been noted in cardiac and skeletal muscle, indicating a potential role in contractile function.\u003cbr\u003e\u003cbr\u003eThe STK33 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, displaying cytoplasmic staining consistent with its kinase activity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=STK33 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STK33 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=STK33 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/STK33 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STK33+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286727021,"sku":"FY12421","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_efa2ab2f-05a1-4ff3-87f9-bfa227895cb0.jpg?v=1782236992","url":"https:\/\/www.ebiohippo.com\/products\/stk33-antibody-serine-threonine-protein-kinase-33-bha17135324","provider":"BioHippo","version":"1.0","type":"link"}