{"product_id":"stx10-antibody-syntaxin-10-bha17135670","title":"STX10 Antibody \/ Syntaxin 10","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSTX10 Antibody \/ Syntaxin 10 is a anti-STX10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Golgi apparatus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e STX10\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSTX10 antibody detects Syntaxin-10, a member of the t-SNARE family involved in vesicle trafficking and membrane fusion within the endosomal system. Encoded by the STX10 gene on chromosome 19q13.32, this protein localizes primarily to the trans-Golgi network (TGN) and endosomes, where it regulates cargo recycling and sorting. Syntaxin-10 interacts with other SNARE proteins, including Vti1a, VAMP3, and STX16, forming complexes that mediate vesicle fusion events critical for protein trafficking between the Golgi apparatus and endosomal compartments.\u003cbr\u003e\u003cbr\u003eSTX10 is essential for maintaining endosomal organization and cargo specificity, particularly in the recycling of mannose 6-phosphate receptors (MPRs) that deliver lysosomal hydrolases. It contributes to the tethering and fusion of vesicles returning from late endosomes to the TGN, a process required for proper lysosome formation and enzyme sorting. Loss of STX10 function disrupts endosomal integrity and leads to defective lysosomal enzyme trafficking, underscoring its role in vesicle-mediated transport.\u003cbr\u003e\u003cbr\u003eThe STX10 antibody is widely used in cell biology, membrane trafficking, and neurobiology research to investigate SNARE-mediated vesicular transport. Western blot analysis identifies a 30 kilodalton band corresponding to Syntaxin-10, while immunofluorescence reveals punctate perinuclear staining that colocalizes with Golgi and endosomal markers such as TGN46 and Rab9. This antibody supports detailed analysis of intracellular trafficking, organelle organization, and vesicle fusion mechanisms.\u003cbr\u003e\u003cbr\u003eBeyond its role in vesicle trafficking, STX10 participates in endosome-to-Golgi transport pathways that maintain protein homeostasis and receptor recycling. It also contributes to specialized trafficking functions in neurons, immune cells, and epithelial tissues. Dysregulation of SNARE complex components, including STX10, has been implicated in neurodegenerative diseases and metabolic disorders where vesicle trafficking pathways are compromised. The STX10 antibody enables researchers to explore these processes and assess Syntaxin-10 function in both normal and pathological conditions.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=STX10 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STX10 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=STX10 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/STX10 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STX10+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047300227437,"sku":"FY12767","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_267c3590-2063-4e06-aa7f-6cb3733f8cd7.jpg?v=1782237038","url":"https:\/\/www.ebiohippo.com\/products\/stx10-antibody-syntaxin-10-bha17135670","provider":"BioHippo","version":"1.0","type":"link"}