{"product_id":"stx4-antibody-syntaxin-4-bha17135818","title":"STX4 Antibody \/ Syntaxin 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSTX4 Antibody \/ Syntaxin 4 is a anti-STX4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane, cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e STX4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSTX4 antibody detects Syntaxin 4, a membrane-anchored SNARE protein that mediates vesicle fusion and exocytosis at the plasma membrane. Encoded by the STX4 gene on chromosome 16p11.2, this t-SNARE protein participates in vesicular trafficking pathways, particularly in insulin-stimulated glucose transporter translocation, neurotransmitter release, and immune cell secretion. Syntaxin 4 plays a central role in membrane docking and fusion by forming SNARE complexes with SNAP23 and VAMP2 or VAMP3.\u003cbr\u003e\u003cbr\u003eStructurally, Syntaxin 4 is a 273-amino-acid membrane protein of approximately 33 kilodaltons containing a C-terminal transmembrane anchor, a SNARE motif required for complex formation, and an N-terminal regulatory domain. It localizes to the plasma membrane and cytoplasmic vesicles in diverse tissues including adipose, neuronal, and epithelial cells. Upon stimulation, Syntaxin 4 assembles into heterotrimeric SNARE complexes that catalyze the fusion of transport vesicles with target membranes.\u003cbr\u003e\u003cbr\u003eThe STX4 antibody is widely used in cell biology, metabolism, and neurophysiology research to study vesicular trafficking, membrane fusion, and regulated exocytosis. Western blot analysis detects a 33 kilodalton band corresponding to Syntaxin 4, while immunofluorescence reveals peripheral and punctate membrane staining. This antibody provides a reliable reagent for analyzing vesicle dynamics and fusion machinery in secretory systems.\u003cbr\u003e\u003cbr\u003eFunctionally, Syntaxin 4 is essential for insulin-dependent GLUT4 trafficking in adipocytes and muscle cells, linking it to glucose uptake and metabolic homeostasis. It also contributes to immune cell secretion of cytokines and cytotoxic granules, as well as neurotransmitter release in the nervous system. Disruption of STX4 impairs exocytic processes and contributes to metabolic disorders such as type 2 diabetes. The STX4 antibody supports studies exploring SNARE-mediated exocytosis, vesicle targeting, and signal-dependent membrane fusion.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=STX4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STX4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=STX4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/STX4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STX4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305896301,"sku":"FY12916","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d1a6cb8a-2007-451a-a27f-18cd43d92a31.jpg?v=1782237050","url":"https:\/\/www.ebiohippo.com\/products\/stx4-antibody-syntaxin-4-bha17135818","provider":"BioHippo","version":"1.0","type":"link"}