{"product_id":"stx5-antibody-syntaxin-5-bha17135407","title":"STX5 Antibody \/ Syntaxin 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSTX5 Antibody \/ Syntaxin 5 is a anti-STX5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Golgi).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e STX5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSTX5 antibody detects Syntaxin-5, a membrane-anchored SNARE protein essential for vesicle trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. Syntaxin-5 mediates membrane fusion events within the early secretory pathway and ensures the proper transport of proteins and lipids through the Golgi complex. The STX5 antibody is widely used in studies of intracellular trafficking, Golgi organization, and protein secretion.\u003cbr\u003e\u003cbr\u003eSyntaxin-5 is encoded by the STX5 gene located on human chromosome 11q12.2. The protein exists as two isoforms generated by alternative translation initiation, a long (42 kilodalton) and short (35 kilodalton) form, both containing an N-terminal coiled-coil region and a C-terminal transmembrane domain. These isoforms localize to different Golgi subcompartments and coordinate SNARE complex formation with partners such as Bet1, Sec22b, and GosR2 to drive vesicle docking and fusion.\u003cbr\u003e\u003cbr\u003eThe STX5 antibody detects a characteristic double band in western blot corresponding to both isoforms. Immunofluorescence typically shows strong perinuclear staining consistent with Golgi localization. Functionally, Syntaxin-5 plays a pivotal role in maintaining Golgi structure and retrograde transport from the Golgi to ER. Disruption of STX5 function leads to ER stress, defective glycosylation, and impaired secretion of transmembrane and secretory proteins.\u003cbr\u003e\u003cbr\u003eSyntaxin-5 also participates in autophagy and lipid metabolism by regulating membrane flow between organelles. Mutations in STX5 cause congenital disorders of glycosylation and multisystemic developmental delay. The protein further contributes to viral replication processes, as certain viruses exploit STX5-dependent pathways to reorganize host membranes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=STX5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STX5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=STX5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/STX5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STX5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290462573,"sku":"FY12504","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f967551a-0a25-44cd-843f-625e2cc14bbf.jpg?v=1782237008","url":"https:\/\/www.ebiohippo.com\/products\/stx5-antibody-syntaxin-5-bha17135407","provider":"BioHippo","version":"1.0","type":"link"}