{"product_id":"stxbp3-antibody-syntaxin-binding-protein-3-bha17136185","title":"STXBP3 Antibody \/ Syntaxin binding protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSTXBP3 Antibody \/ Syntaxin binding protein 3 is a anti-STXBP3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e STXBP3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSTXBP3 antibody recognizes Syntaxin binding protein 3, a member of the Sec1\/Munc18-like (SM) family of proteins that regulate vesicle fusion and exocytosis. The STXBP3 gene encodes a cytosolic protein also known as Munc18c, which interacts with syntaxins, particularly Syntaxin 4, to mediate docking and fusion of vesicles with the plasma membrane. This interaction is critical for regulated exocytosis in diverse cell types including adipocytes, pancreatic beta cells, neurons, and epithelial cells. By controlling SNARE complex assembly, STXBP3 ensures proper timing of vesicle release during insulin secretion, neurotransmission, and cell-surface trafficking of key receptors and transporters.\u003cbr\u003e\u003cbr\u003eSTXBP3 plays a central role in glucose homeostasis. In adipose and muscle tissue, Munc18c regulates insulin-stimulated translocation of glucose transporter 4 (GLUT4) to the plasma membrane, an essential step in glucose uptake. Dysregulation or reduced expression of STXBP3 has been associated with insulin resistance and type 2 diabetes. In pancreatic beta cells, STXBP3 interacts with Syntaxin 1A and VAMP2 to influence insulin granule exocytosis. Beyond metabolic regulation, STXBP3 participates in vesicle trafficking in epithelial tissues and contributes to cell polarity maintenance and junctional organization.\u003cbr\u003e\u003cbr\u003eThe human STXBP3 gene is located on chromosome 1p36.23 and contains multiple alternatively spliced isoforms. Protein structure analysis reveals that STXBP3 comprises three domains that form a closed conformation around its syntaxin partners, preventing premature SNARE complex formation. This inhibitory mechanism ensures vesicles only fuse in response to proper signaling cues. The protein's expression is ubiquitous but enriched in secretory tissues and endocrine glands. STXBP3 has also been implicated in immune responses through regulation of vesicle-mediated cytokine release.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining with STXBP3 antibody demonstrates cytoplasmic localization in skeletal muscle, pancreatic islets, and epithelial cells. It serves as a valuable reagent for studies of insulin signaling, exocytosis, vesicular transport, and metabolic regulation. The STXBP3 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=STXBP3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=STXBP3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=STXBP3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/STXBP3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=STXBP3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320150381,"sku":"FY13283","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_02fd2c7e-64b7-4982-89c1-e99044107049.jpg?v=1782237105","url":"https:\/\/www.ebiohippo.com\/products\/stxbp3-antibody-syntaxin-binding-protein-3-bha17136185","provider":"BioHippo","version":"1.0","type":"link"}