{"product_id":"sun5-antibody-sad1-and-unc84-domain-containing-protein-5-bha17136009","title":"SUN5 Antibody \/ Sad1 and UNC84 domain-containing protein 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSUN5 Antibody \/ Sad1 and UNC84 domain-containing protein 5 is a anti-SUN5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SUN5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSUN5 antibody detects Sad1 and UNC84 domain-containing protein 5, a nuclear envelope protein that anchors the nucleus to the cytoskeleton during spermatogenesis. The UniProt recommended name is Sad1 and UNC84 domain-containing protein 5 (SUN5). This transmembrane protein is part of the linker of nucleoskeleton and cytoskeleton (LINC) complex, which connects nuclear components to cytoskeletal filaments, ensuring nuclear positioning and structural integrity.\u003cbr\u003e\u003cbr\u003eFunctionally, SUN5 antibody identifies a 379-amino-acid protein localized to the inner nuclear membrane, where it interacts with outer membrane proteins such as Nesprins through its conserved SUN domain. SUN5 is particularly enriched in spermatids, where it mediates head-to-tail coupling during sperm maturation. Loss of SUN5 disrupts this linkage, leading to acephalic spermatozoa and infertility.\u003cbr\u003e\u003cbr\u003eThe SUN5 gene is located on chromosome 20q11.22 and is predominantly expressed in testis, with lower expression in other tissues. Its testis-specific expression pattern underscores its specialized function in spermiogenesis. SUN5 contributes to nuclear shaping, centrosome attachment, and flagellar organization through mechanical coupling between the nucleus and cytoskeleton.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in SUN5 are associated with acephalic spermatozoa syndrome and male infertility due to defective sperm head-tail junction formation. Research using SUN5 antibody supports studies in reproductive biology, nuclear envelope architecture, and cytoskeletal interaction mechanisms.\u003cbr\u003e\u003cbr\u003eSUN5 antibody is validated for use in western blotting, immunofluorescence, and immunohistochemistry to study LINC complex components and spermatid structure. \u003cbr\u003e\u003cbr\u003eStructurally, SUN5 contains a coiled-coil region and a conserved C-terminal SUN domain that mediates interaction with KASH domain proteins across the nuclear envelope. This antibody helps elucidate SUN5's molecular function in nuclear-cytoskeletal attachment and fertility-related disorders.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SUN5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SUN5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SUN5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SUN5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SUN5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312384365,"sku":"FY13107","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4f4ac503-5f30-418b-b298-e53ce844bcf6.jpg?v=1782237100","url":"https:\/\/www.ebiohippo.com\/products\/sun5-antibody-sad1-and-unc84-domain-containing-protein-5-bha17136009","provider":"BioHippo","version":"1.0","type":"link"}