{"product_id":"superlight-atto-hrp-reagent-bht15600253","title":"SuperLight™ Atto HRP Reagent","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor HRP determination in ELISA, Southern Blot, Western Blot. The assay uses Luminescence for signal readout. Compatible sample input includes horse-radish peroxidase and its conjugates. Typical stated assay timing is 10 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e Luminescence supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes horse-radish peroxidase and its conjugates, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of Atto- to low femtomolar for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Single reagent: Single, stable, and ready to use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight High sensitivity: Detection of HRP in the atto- to low femtogram range. Available format information for this listing includes 200 Tests in 96-well plate (800 Tests in 384-well plate).\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of superlight atto hrp within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003ePEROXIDASES\u003c\/i\u003e(or peroxide reductases, EC number 1.11.1.x) are a large group of enzymes that break up peroxides and play important roles in various biological processes. Horse Radish Peroxidase (HRP) is an enzyme commonly used for conjugation. One example is antibody-HRP conjugate that is frequently and widely used in immunoassays such as ELISA and Western blots. BioAssay Systems’ SuperLight™ Atto HRP Reagent provides an ultra-sensitive luminescent means for detecting HRP activity and its conjugates. The improved reagent rapidly reacts with peroxidase or the HRP in an HRP-conjugate, producing highly stable and intense luminescence for maximum detection of the peroxidase activity in solution assays.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eLuminescence.\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: Atto- to low femtomolar.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 10 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n  \u003cli\u003eMatched standards, blanks, and replicate wells are typically used to improve interpretability across batches and sample matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePrepare control-treated samples for paired use with compatible assay-kit plates.\u003c\/li\u003e\n  \u003cli\u003eBenchmark assay response against defined control conditions in replicate wells.\u003c\/li\u003e\n  \u003cli\u003eOptimize reagent concentration and incubation windows for plate-based comparisons.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"200 Tests in 96-well plate (800 Tests in 384-well plate)","offer_id":53238320365933,"sku":"SLAP-200","price":125.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/SLAPfig.jpg?v=1776668363","url":"https:\/\/www.ebiohippo.com\/products\/superlight-atto-hrp-reagent-bht15600253","provider":"BioHippo","version":"1.0","type":"link"}