{"product_id":"supt20h-antibody-transcription-factor-spt20-homolog-bha17135742","title":"SUPT20H Antibody \/ Transcription factor SPT20 homolog","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSUPT20H Antibody \/ Transcription factor SPT20 homolog is a anti-SUPT20H Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SUPT20H\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSUPT20H antibody detects Transcription factor SPT20 homolog, a multifunctional protein involved in transcriptional regulation, chromatin remodeling, and proteostasis. Encoded by the SUPT20H gene on chromosome 13q12.3, this evolutionarily conserved factor plays dual roles as a component of transcriptional coactivator complexes and as a regulator of protein homeostasis in the cytoplasm. SUPT20H participates in the regulation of RNA polymerase II-mediated transcription, chromatin accessibility, and cellular stress response.\u003cbr\u003e\u003cbr\u003eStructurally, SUPT20H contains conserved coiled-coil and low-complexity regions that facilitate interactions with chromatin-associated proteins, transcription factors, and the proteasome. It is a functional homolog of yeast SPT20, a core subunit of the SAGA (Spt-Ada-Gcn5 acetyltransferase) transcriptional coactivator complex that links histone acetylation to gene activation. In mammalian systems, SUPT20H contributes to nucleosome remodeling and transcriptional coactivation by coupling histone modification with recruitment of RNA polymerase II at promoter regions.\u003cbr\u003e\u003cbr\u003eThe SUPT20H antibody is widely used in epigenetics, gene regulation, and cell biology research to study transcriptional activation, chromatin remodeling, and stress adaptation. Western blot analysis detects a 100 kilodalton band corresponding to SUPT20H, while immunofluorescence reveals both nuclear and cytoplasmic staining, reflecting its multifunctional localization. This antibody enables researchers to investigate how chromatin dynamics and transcriptional machinery coordinate gene expression in response to cellular cues.\u003cbr\u003e\u003cbr\u003eBeyond its nuclear role, SUPT20H interacts with the proteasome and heat shock proteins to regulate protein degradation and quality control under stress conditions. It assists in clearing misfolded proteins and maintaining cytoplasmic proteostasis. Dysregulation of SUPT20H expression or mutations affecting its protein-interaction motifs have been linked to altered transcriptional fidelity, stress sensitivity, and oncogenic transformation. The SUPT20H antibody provides a powerful tool for characterizing these processes at the molecular level.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SUPT20H - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SUPT20H - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SUPT20H - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SUPT20H - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SUPT20H+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303602541,"sku":"FY12840","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d9bb329e-76e7-46c1-a50e-cf4148832a26.jpg?v=1782237056","url":"https:\/\/www.ebiohippo.com\/products\/supt20h-antibody-transcription-factor-spt20-homolog-bha17135742","provider":"BioHippo","version":"1.0","type":"link"}