{"product_id":"surf6-antibody-surfeit-locus-protein-6-bha17136111","title":"SURF6 Antibody \/ Surfeit locus protein 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSURF6 Antibody \/ Surfeit locus protein 6 is a anti-SURF6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nucleus, Nucleolus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SURF6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSURF6 antibody detects Surfeit locus protein 6, a nucleolar protein involved in ribosome biogenesis and pre-rRNA processing. The UniProt recommended name is Surfeit locus protein 6 (SURF6). This evolutionarily conserved nucleolar factor functions as a scaffold for ribonucleoprotein complex assembly during early steps of ribosome formation.\u003cbr\u003e\u003cbr\u003eFunctionally, SURF6 antibody identifies a 360-amino-acid protein localized to the granular component of the nucleolus, where it associates with pre-rRNA transcripts and ribosomal assembly intermediates. SURF6 interacts with fibrillarin and nucleolin, facilitating pre-rRNA maturation and 60S ribosomal subunit formation. Its RNA-binding capacity and nucleolar localization signal make it an essential factor for ribosome production and nucleolar organization.\u003cbr\u003e\u003cbr\u003eThe SURF6 gene is located on chromosome 9q34.2 and is expressed ubiquitously, with high levels in proliferating and metabolically active cells. Expression correlates with cellular growth rate, reflecting its role in ribosome synthesis and protein translation capacity.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of SURF6 expression has been linked to oncogenesis and stress response modulation. Overexpression is observed in various cancers and is associated with increased ribosome biogenesis and cell proliferation. Conversely, depletion of SURF6 triggers nucleolar stress, p53 activation, and growth arrest. Research using SURF6 antibody supports studies in ribosome assembly, nucleolar dynamics, and cancer biology.\u003cbr\u003e\u003cbr\u003eSURF6 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect nucleolar proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Surfeit locus protein 6 contains arginine-rich motifs that mediate RNA binding and multiple acidic stretches that promote protein-protein interactions within the nucleolus. Its modular domains contribute to the architecture of the ribosomal assembly machinery. This antibody facilitates exploration of SURF6's function in nucleolar integrity and ribosome maturation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SURF6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SURF6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SURF6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SURF6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SURF6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318184301,"sku":"FY13209","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3d084cb8-421d-42f6-bfad-c574d1c72c7c.jpg?v=1782237068","url":"https:\/\/www.ebiohippo.com\/products\/surf6-antibody-surfeit-locus-protein-6-bha17136111","provider":"BioHippo","version":"1.0","type":"link"}