{"product_id":"sycn-antibody-syncollin-bha17135758","title":"SYCN Antibody \/ Syncollin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSYCN Antibody \/ Syncollin is a anti-SYCN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SYCN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSYCN antibody detects Syncollin, a secretory vesicle membrane protein that plays an important role in pancreatic enzyme secretion and zymogen granule exocytosis. Encoded by the SYCN gene on chromosome 19q13.33, Syncollin is a small protein localized to the membranes of secretory vesicles within pancreatic acinar cells and other exocrine tissues. It functions as an accessory factor that promotes granule maturation, docking, and fusion with the plasma membrane during regulated secretion.\u003cbr\u003e\u003cbr\u003eStructurally, Syncollin contains a coiled-coil domain responsible for oligomerization and membrane tethering, allowing it to form homo-oligomeric complexes on the luminal surface of zymogen granules. These complexes interact with SNARE proteins and other components of the exocytic machinery to facilitate vesicle fusion and enzyme release. SYCN contributes to the organization of zymogen granule membranes and ensures precise regulation of digestive enzyme secretion in response to cholecystokinin and other secretagogues.\u003cbr\u003e\u003cbr\u003eThe SYCN antibody is widely used in cell biology, gastrointestinal physiology, and secretion research to investigate vesicle dynamics, exocytosis, and pancreatic function. Western blot analysis detects a 13 kilodalton band corresponding to Syncollin, while immunofluorescence reveals punctate staining in the apical cytoplasm of acinar cells and along the zymogen granule membranes. This antibody supports analysis of regulated secretory pathways and granule maturation in exocrine tissues.\u003cbr\u003e\u003cbr\u003eDisruption or reduced expression of SYCN impairs vesicle exocytosis and digestive enzyme secretion, leading to altered pancreatic physiology. In experimental models, loss of Syncollin function results in defective granule docking and diminished amylase release, highlighting its essential role in the secretory machinery. The SYCN antibody provides a robust reagent for examining vesicle organization, exocytotic mechanisms, and the structural biology of secretory organelles.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SYCN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SYCN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SYCN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SYCN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SYCN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303766381,"sku":"FY12856","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9e72b6f7-a0fc-41f3-bdd3-198047974f2b.jpg?v=1782237053","url":"https:\/\/www.ebiohippo.com\/products\/sycn-antibody-syncollin-bha17135758","provider":"BioHippo","version":"1.0","type":"link"}