{"product_id":"syf2-antibody-pre-mrna-splicing-factor-syf2-bha17135675","title":"SYF2 Antibody \/ Pre-mRNA splicing factor SYF2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSYF2 Antibody \/ Pre-mRNA splicing factor SYF2 is a anti-SYF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SYF2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSYF2 antibody detects Pre-mRNA splicing factor SYF2, a component of the spliceosome complex that participates in mRNA processing, cell cycle progression, and DNA damage response. Encoded by the SYF2 gene on chromosome 1p35.2, this protein acts as a conserved splicing factor that associates with the U5 snRNP complex and other splicing regulators. SYF2 is required for efficient assembly and catalysis of the spliceosome, ensuring accurate intron removal and exon joining during pre-mRNA maturation. Its function extends beyond RNA processing to cell proliferation and genomic stability.\u003cbr\u003e\u003cbr\u003eSYF2 interacts with PRPF19, CDC5L, and other components of the spliceosome-associated NTC complex to facilitate transition between spliceosome conformations. It also coordinates splicing with transcription by associating with RNA polymerase II elongation complexes. In addition to its role in RNA processing, SYF2 is involved in G1\/S phase progression, likely through regulation of splicing of genes involved in DNA replication and repair. Depletion of SYF2 disrupts normal cell cycle progression, underscoring its role in proliferative control.\u003cbr\u003e\u003cbr\u003eThe SYF2 antibody is used in RNA biology, cell cycle, and genomics research to study spliceosome assembly and mRNA maturation. Western blot analysis identifies a 24 kilodalton band corresponding to SYF2, and immunofluorescence demonstrates nuclear localization consistent with its splicing function. This antibody enables monitoring of SYF2 expression and localization in studies of transcriptional regulation and RNA processing.\u003cbr\u003e\u003cbr\u003eIn cancer research, SYF2 has emerged as a proliferation-associated factor, with overexpression reported in breast, lung, and colorectal cancers. It may contribute to tumor progression by promoting efficient splicing of growth-related transcripts. The SYF2 antibody provides a reliable tool for exploring splicing factor regulation, cell cycle control, and their intersections in disease mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SYF2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SYF2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SYF2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SYF2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SYF2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299309933,"sku":"FY12772","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_24d0d703-23d7-4a13-a24e-d3bad34379a3.jpg?v=1782237038","url":"https:\/\/www.ebiohippo.com\/products\/syf2-antibody-pre-mrna-splicing-factor-syf2-bha17135675","provider":"BioHippo","version":"1.0","type":"link"}