{"product_id":"syne3-antibody-nesprin-3-bha17135854","title":"SYNE3 Antibody \/ Nesprin 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSYNE3 Antibody \/ Nesprin 3 is a anti-SYNE3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30S37 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SYNE3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30S37, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSYNE3 antibody detects Nesprin 3, encoded by the SYNE3 gene. This protein belongs to the spectrin repeat containing nuclear envelope protein family and is a component of the LINC complex, which connects the nuclear envelope to the cytoskeleton. Unlike other family members that link directly to actin filaments, Nesprin 3 connects to intermediate filaments through partners such as plectin, providing a unique bridge between the nucleus and cytoskeletal structures. Researchers use SYNE3 antibody to investigate nuclear positioning, mechanotransduction, and the maintenance of structural integrity across diverse cell types.\u003cbr\u003e\u003cbr\u003eThe LINC complex serves as a conduit for mechanical signals transmitted between the cytoplasm and the nucleus. By anchoring the nuclear envelope to cytoskeletal elements, Nesprin3 helps stabilize nuclear shape and position during migration, mitosis, and differentiation. When detected with SYNE3 antibody, changes in its expression or distribution can be correlated with altered mechanosensing and transcriptional regulation. These processes are essential for proper tissue development and for cellular responses to physical stress, making the study of SYNE3 protein highly relevant to mechanobiology.\u003cbr\u003e\u003cbr\u003eDysregulation of nuclear envelope proteins contributes to a variety of diseases, including muscular dystrophies, cardiomyopathies, and neurological disorders. Although more attention has historically focused on Nesprin1 and Nesprin2, studies suggest that Nesprin 3 also participates in these pathways by weakening nuclear anchorage when perturbed. SYNE3 antibody enables detection of protein alterations in both healthy and diseased tissue, providing a means to assess its contribution to pathogenesis. Evidence has also linked SYNE3 to cancer progression, where nuclear deformability affects metastatic cell migration through dense tissue matrices.\u003cbr\u003e\u003cbr\u003eSYNE3 antibody is widely used in western blotting, immunofluorescence, and immunohistochemistry. In western blotting, it detects protein levels across tissue lysates, while immunofluorescence confirms its nuclear envelope localization in cultured cells. Immunohistochemistry highlights expression patterns in tissues such as muscle, skin, and epithelium, reflecting its role in nuclear architecture. Functional studies using SYNE3 antibody in knockdown and rescue experiments have demonstrated how loss of the protein disrupts nuclear positioning and cell motility, reinforcing its role in structural and signaling networks.\u003cbr\u003e\u003cbr\u003eBy providing validated SYNE3 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SYNE3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SYNE3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SYNE3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SYNE3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SYNE3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047307403629,"sku":"FY12952","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a9fedf4e-0b0d-4912-97dc-8bc220d711c7.jpg?v=1772019368","url":"https:\/\/www.ebiohippo.com\/products\/syne3-antibody-nesprin-3-bha17135854","provider":"BioHippo","version":"1.0","type":"link"}