{"product_id":"synm-antibody-synemin-bha17135249","title":"SYNM Antibody \/ Synemin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSYNM Antibody \/ Synemin is a anti-SYNM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SYNM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SYNM antibody targets Synemin, a large intermediate filament-associated protein encoded by the SYNM gene. Synemin belongs to the intermediate filament (IF) protein family and serves as a cytoskeletal crosslinker that connects desmin and vimentin filaments to cellular structures. Through these interactions, Synemin helps maintain cellular architecture, mechanical stability, and signaling dynamics. The SYNM antibody allows precise detection of Synemin to study cytoskeletal organization and mechanotransduction in muscle and non-muscle cells.\u003cbr\u003e\u003cbr\u003eSynemin exists in multiple isoforms, including alpha and beta forms, which differ in size and tissue distribution. The alpha isoform is primarily expressed in striated muscle, while the beta isoform occurs in smooth muscle and non-muscle tissues such as astrocytes and hepatocytes. Both isoforms contain a conserved intermediate filament�binding domain that mediates filament anchoring and a unique C-terminal tail that interacts with cytoskeletal and signaling proteins. The SYNM antibody detects these isoforms, enabling evaluation of Synemin expression across tissues and developmental stages.\u003cbr\u003e\u003cbr\u003eFunctionally, Synemin acts as a structural scaffold linking intermediate filaments to focal adhesions, costameres, and the plasma membrane. It binds to alpha-actinin, dystrophin, and protein kinase A regulatory subunits, integrating cytoskeletal mechanics with signal transduction. Loss or mutation of Synemin disrupts cell shape, elasticity, and contractility, emphasizing its role in maintaining structural integrity. Researchers employ the SYNM antibody to characterize these cytoskeletal connections and assess their impact on tissue strength and cellular signaling.\u003cbr\u003e\u003cbr\u003eSynemin expression is prominent in cardiac and skeletal muscle, where it contributes to sarcomere alignment and force transmission. Dysregulation of Synemin may contribute to muscular dystrophies, cardiomyopathies, and other myopathic conditions. The SYNM antibody supports these investigations by providing a consistent and specific reagent for identifying Synemin in muscle biopsies, cultured myocytes, and tissue sections. Beyond muscle, Synemin has been implicated in glial and epithelial cell structure, suggesting roles in neurobiology and cancer progression.\u003cbr\u003e\u003cbr\u003eExperimental applications of the SYNM antibody include western blotting, immunofluorescence, and immunohistochemistry. These assays reveal Synemin's filamentous distribution and dynamic rearrangement during cell migration or mechanical stress.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SYNM - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SYNM - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SYNM - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SYNM - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SYNM+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284957549,"sku":"FY12346","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e5751432-89b5-4802-a55e-4944134e2721.jpg?v=1782237008","url":"https:\/\/www.ebiohippo.com\/products\/synm-antibody-synemin-bha17135249","provider":"BioHippo","version":"1.0","type":"link"}