{"product_id":"syt10-antibody-synaptotagmin-10-bha17135848","title":"SYT10 Antibody \/ Synaptotagmin 10","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSYT10 Antibody \/ Synaptotagmin 10 is a anti-SYT10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane, vesicles.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SYT10\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSYT10 antibody detects Synaptotagmin-10, a member of the synaptotagmin family of membrane-trafficking proteins involved in calcium-dependent vesicle exocytosis. The UniProt recommended name is Synaptotagmin-10 (SYT10), with alternate names including Synaptotagmin X and p65. Synaptotagmins act as calcium sensors that regulate synaptic vesicle fusion with the plasma membrane, mediating neurotransmitter and hormone release. SYT10, like other synaptotagmins, contains two C2 domains that bind calcium and phospholipids to trigger vesicle exocytosis.\u003cbr\u003e\u003cbr\u003eFunctionally, SYT10 antibody identifies a 565-amino-acid protein that plays a role in the regulation of insulin secretion and neuronal exocytosis. SYT10 is unique among synaptotagmins because it is expressed in both neuronal and non-neuronal tissues, including the pancreas and pituitary gland. It is associated with the release of insulin-like growth factor 1 (IGF-1) and other neuropeptides in a calcium-dependent manner. Through its C2A and C2B domains, SYT10 mediates the calcium-triggered docking and fusion of secretory vesicles, ensuring precise timing of exocytosis following intracellular signaling events.\u003cbr\u003e\u003cbr\u003eThe SYT10 antibody is used to study secretory pathways, vesicular transport, and neuronal communication. In neurons, SYT10 localizes to presynaptic membranes and regulates neurotransmitter release by acting as a Ca2+-sensor for synaptic vesicle fusion. In endocrine cells, it supports hormone secretion by coupling calcium signaling to vesicular exocytosis. Expression of SYT10 is enriched in specific brain regions such as the cortex and cerebellum, reflecting specialized regulatory roles in neurotransmission.\u003cbr\u003e\u003cbr\u003eMutations or dysregulation of SYT10 have been associated with altered synaptic signaling and insulin secretion. The SYT10 gene is located on chromosome 12q21.32 and encodes a protein with two conserved C2 domains responsible for calcium and phospholipid binding. Unlike some synaptotagmins that promote rapid synchronous release, SYT10 is implicated in sustained and asynchronous release events. Research indicates that SYT10 may also participate in neurotrophic signaling and membrane repair processes.\u003cbr\u003e\u003cbr\u003eSYT10 antibody applications include western blotting, immunocytochemistry, and confocal microscopy to examine vesicle trafficking, synaptic plasticity, and calcium-triggered secretion. Its use extends to neuroendocrine and pancreatic models where SYT10 mediates regulated exocytosis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SYT10 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SYT10 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SYT10 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SYT10 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SYT10+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307665773,"sku":"FY12946","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c73d9ad9-b6a1-44f3-a8db-f151e217fbfe.jpg?v=1782237069","url":"https:\/\/www.ebiohippo.com\/products\/syt10-antibody-synaptotagmin-10-bha17135848","provider":"BioHippo","version":"1.0","type":"link"}