{"product_id":"syt12-antibody-synaptotagmin-12-bha17135473","title":"SYT12 Antibody \/ Synaptotagmin 12","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSYT12 Antibody \/ Synaptotagmin 12 is a anti-SYT12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SYT12\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSYT12 antibody detects Synaptotagmin 12, a member of the synaptotagmin family of membrane-trafficking proteins that regulate neurotransmitter release and synaptic vesicle exocytosis. Unlike calcium-sensing synaptotagmins, SYT12 lacks functional calcium-binding domains but modulates synaptic transmission by controlling vesicle docking and fusion probability. The SYT12 antibody is widely used in neurobiology and synaptic physiology to study vesicle release mechanisms, synaptic modulation, and neuronal signaling.\u003cbr\u003e\u003cbr\u003eSYT12 is encoded by the SYT12 gene located on human chromosome 11q12.1. The protein is approximately 240 amino acids long and contains two C2-like domains typical of the synaptotagmin family. However, substitutions in key residues render these domains calcium-insensitive, distinguishing SYT12 as a regulator of synaptic machinery rather than a classical calcium sensor. SYT12 localizes predominantly to presynaptic terminals and associates with SNARE complex components that drive vesicle fusion.\u003cbr\u003e\u003cbr\u003eAn SYT12 antibody detects a 28 kilodalton band by western blot and reveals punctate staining at presynaptic sites under confocal microscopy. SYT12 interacts with SNAP25 and syntaxin, modulating the assembly of the SNARE complex and influencing vesicle priming efficiency. Experimental silencing of SYT12 in neurons decreases spontaneous and evoked neurotransmitter release, indicating its role in maintaining basal synaptic transmission.\u003cbr\u003e\u003cbr\u003eBeyond synaptic function, SYT12 participates in hormone secretion and calcium-independent vesicle trafficking in endocrine cells. Mutations in SYT12 have been linked to neurological and psychiatric conditions, including epilepsy and movement disorders. In cancer research, SYT12 overexpression correlates with poor prognosis in lung and colorectal cancers, where it enhances tumor cell migration through altered vesicle dynamics.\u003cbr\u003e\u003cbr\u003eSYT12 exemplifies the regulatory diversity of synaptotagmin family proteins, balancing vesicle fusion without directly sensing calcium.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SYT12 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SYT12 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SYT12 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SYT12 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SYT12+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292166509,"sku":"FY12570","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b4f96780-7213-4706-97e3-2e9b6a1afe17.jpg?v=1782237052","url":"https:\/\/www.ebiohippo.com\/products\/syt12-antibody-synaptotagmin-12-bha17135473","provider":"BioHippo","version":"1.0","type":"link"}