{"product_id":"t3re-dr4-reporter-lentivirus-bhv19400046","title":"T3RE\/DR4 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe thyroid hormone response element arranged as a direct repeat spaced by four nucleotides (T3RE\/DR4) is the DNA binding site recognized by thyroid hormone receptors. These nuclear receptors heterodimerize with the retinoid X receptor and, upon binding the active thyroid hormone triiodothyronine (T3), switch from transcriptional repression to activation of target genes. Thyroid hormone signaling regulates basal metabolic rate, growth, cardiac function, and neural development. In this system thyroid stimulating hormone receptor (TSHR) signaling drives T3-dependent transcription through the DR4 element, providing a readout of thyroid pathway activity. Dysregulated thyroid hormone signaling underlies hypothyroidism, hyperthyroidism, and metabolic disease, making this pathway important in endocrinology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe T3RE\/DR4 Reporter Lentivirus is a transcription factor reporter system that delivers a quantitative readout of thyroid hormone receptor pathway activity in mammalian cells. The construct places a fluorescent or luminescent reporter (GFP, RFP, or firefly luciferase) under the control of tandem consensus DR4 response elements coupled to a minimal promoter and an optimized upstream mini-enhancer that amplifies signal-to-noise. A constitutive drug selection marker (Puromycin or Blasticidin) enables straightforward establishment of stable reporter cell lines. Stable lentiviral integration provides consistent reporter expression and supports readout by fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is suited to studying thyroid hormone responses in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, mCherry, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Thyroid stimulating hormone receptor (TSHR) pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251625845101,"sku":"LTV-0050-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310892048749,"sku":"LTV-0050-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310892081517,"sku":"LTV-0050-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310892114285,"sku":"LTV-0050-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310892147053,"sku":"LTV-0050-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310892179821,"sku":"LTV-0050-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310892212589,"sku":"LTV-0050-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_d9e4e2d0-4706-4db9-8585-54d4313caa22.jpg?v=1776934148","url":"https:\/\/www.ebiohippo.com\/products\/t3re-dr4-reporter-lentivirus-bhv19400046","provider":"BioHippo","version":"1.0","type":"link"}