{"product_id":"t5-exonuclease-10-u-mu-l-bhz20800096","title":"T5 Exonuclease (10 U\/μL)","description":"\u003cp class=\"p\"\u003e\u003cspan\u003eT5 Exonuclease is a double-stranded DNA-specific exonuclease and single-stranded DNA endonuclease. It can initiate digestion from the 5' end of single- or double-stranded DNA, as well as from gaps or nicks in linear or circular double-stranded DNA, but it does not degrade supercoiled double-stranded DNA. Therefore, T5 Exonuclease is widely used for removing incompletely ligated products from circularized dsDNA, degrading denatured DNA in alkaline extraction plasmids, degrading linear and nicked DNA in plasmid samples, and seamless cloning (Gibson assembly), etc.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eApplications\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eIn-Fusion Cloning\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eDegradation and alkaline lysis extraction of denatured DNA from plasmids\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eSpecifications\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"207\"\u003e\n\u003cp class=\"MsoNormal\"\u003eConcentration\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"434\"\u003e\n\u003cp class=\"MsoNormal\"\u003e10 U\/µL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"207\"\u003e\n\u003cp class=\"MsoNormal\"\u003eUnit Definition\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"434\"\u003e\n\u003cp class=\"MsoNormal\"\u003eOne unit is defined as the amount of enzyme required to effect a change of 0.00032 A260 nm per minute at 37°C in the buffer\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eComponents\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" class=\"MsoTableGrid\" style=\"width: 100.003%; height: 107.546px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.6019px;\"\u003e\n\u003ctd style=\"width: 25.2236%; height: 35.6019px;\" valign=\"top\" width=\"141\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ca name=\"_Hlk184733933\"\u003e\u003c\/a\u003e\u003c\/span\u003eComponents No.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 38.8193%; height: 35.6019px;\" valign=\"top\" width=\"217\"\u003e\n\u003cp class=\"MsoNormal\"\u003eName\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 18.4258%; height: 35.6019px;\" valign=\"top\" width=\"103\"\u003e\n\u003cp class=\"MsoNormal\"\u003e14538ES80\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 16.1002%; height: 35.6019px;\" valign=\"top\" width=\"90\"\u003e\n\u003cp class=\"MsoNormal\"\u003e14538ES90\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36.3426px;\"\u003e\n\u003ctd style=\"width: 25.2236%; height: 36.3426px;\" valign=\"top\" width=\"141\"\u003e\n\u003cp class=\"MsoNormal\"\u003e14538-A\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 38.8193%; height: 36.3426px;\" valign=\"center\" width=\"217\"\u003e\n\u003cp class=\"MsoNormal\"\u003eT5 Exonuclease (10 U\/µL)\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 18.4258%; height: 36.3426px;\" valign=\"center\" width=\"103\"\u003e\n\u003cp class=\"MsoNormal\"\u003e100 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 16.1002%; height: 36.3426px;\" valign=\"center\" width=\"90\"\u003e\n\u003cp class=\"MsoNormal\"\u003e500 μL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.6019px;\"\u003e\n\u003ctd style=\"width: 25.2236%; height: 35.6019px;\" valign=\"top\" width=\"141\"\u003e\n\u003cp class=\"MsoNormal\"\u003e14538-B\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 38.8193%; height: 35.6019px;\" valign=\"center\" width=\"217\"\u003e\n\u003cp class=\"MsoNormal\"\u003eT5 Exonuclease Buffer(10×）\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 18.4258%; height: 35.6019px;\" valign=\"center\" width=\"103\"\u003e\n\u003cp class=\"MsoNormal\"\u003e1 mL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 16.1002%; height: 35.6019px;\" valign=\"center\" width=\"90\"\u003e\n\u003cp class=\"MsoNormal\"\u003e1 mL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eShipping and Storage\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003eThe products should be stored at -25℃ ~ -15℃ for 3 years.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eFigures\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" style=\"text-align: center;\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_9ab58984-8da1-48a9-9249-dd98fd64e1dc.png?v=1736821255\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eFigure1. Cloning Situation of Different Fragment Connections\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003eNote: In-Fusion Cloning was carried out using T5 Exonuclease, with the connection of 2, 3, and 4 fragments respectively. The experimental results demonstrate that the application of T5 Exonuclease in In-Fusion Cloning can achieve a 100% positive clone rate (12 out of 12)\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"5000 U","offer_id":53294151205229,"sku":"14538ES90","price":265.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_9ab58984-8da1-48a9-9249-dd98fd64e1dc.png?v=1778101062","url":"https:\/\/www.ebiohippo.com\/products\/t5-exonuclease-10-u-mu-l-bhz20800096","provider":"BioHippo","version":"1.0","type":"link"}