{"product_id":"t7-rna-polymerase-gmp-grade-250-u-mu-l-bhz20800044","title":"T7 RNA Polymerase GMP-grade (250 U\/μL)","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\n\u003cp\u003eThis product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA.\u003c\/p\u003e\n\u003cp\u003eNote: G* is the first base of the RNA transcript.\u003c\/p\u003e\n\u003cp\u003eThis product is produced in accordance with GMP regulations and provided in liquid form.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSynthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template\u003c\/li\u003e\n\u003cli\u003eHigher yield and easy to operate\u003c\/li\u003e\n\u003cli\u003eLower endotoxins\u003c\/li\u003e\n\u003cli\u003eTested for the absence of endonucleases, exonucleases, RNases\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRadiolabeled RNA probe preparation\u003c\/li\u003e\n\u003cli\u003eRNA generation for studies of RNA structure, processing and catalysis\u003c\/li\u003e\n\u003cli\u003eExpression control via anti-sense RNA\u003c\/li\u003e\n\u003cli\u003emRNA, sgRNA synthesis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSource\u003c\/td\u003e\n\u003ctd\u003eExpressed in\u003cem\u003e E. coli\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003ewith a recombinant T7 RNA Polymerase gene \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptimum Temperature\u003c\/td\u003e\n\u003ctd\u003e37℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v\/v) glycerin，pH7.9 at 25℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eThe amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\n\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\n\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003eQuality\u003c\/h2\u003e\n\u003ctable width=\"1089\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eCOA Items\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eStandard\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eActivity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e250-300U\/μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\n\u003cspan\u003eProtein\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003econcentration\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e≥0.5mg\/mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e≥95%\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eEndotoxin\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e\u0026lt;20 EU\/mg\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eResidual Protease\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eResidual Exonuclease\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eResidual Nickase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eResidual RNase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\n\u003cspan\u003eE.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecoli host protein\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e\u0026lt;50ppm\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\n\u003cspan\u003eE.coli host\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eDNA\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003e\u0026lt;10 fg\/U\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 350.354px;\" width=\"239\"\u003e\u003cspan\u003eMycoplasma DNA\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 180.979px;\" width=\"850\"\u003e\u003cspan\u003eNegative\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 class=\"H2_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponents No.\u003c\/td\u003e\n\u003ctd\u003eName\u003c\/td\u003e\n\u003ctd\u003e10625ES10 (10 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES60 (100 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES86 (2,500 KU)\u003c\/td\u003e\n\u003ctd\u003e10625ES99 (100 MU)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10625\u003c\/td\u003e\n\u003ctd\u003eT7 RNA Polymerase GMP-grade (250 U\/μL)\u003c\/td\u003e\n\u003ctd\u003e40 μL\u003c\/td\u003e\n\u003ctd\u003e400 μL\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003ctd\u003e400 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003eT7 RNA Polymerase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cspan\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/693866\/322e-167030612134953504.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/seas.ysbuy.com\/file\/98179\/8309-167030624349998928.png\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigures 2. The transcription demonstration of different lengths RNA by T7 kit respectively in the electrophoretogram (Figures 2A), the capillary electrophoresis diagram (Figures 2B), and the chromatogram (Figures 2C)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0803\/9419\/1166\/files\/T7_480x480.png?v=1700873194\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 3. Synthesis of capped RNA in vitro.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 3A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 3B.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10 KU","offer_id":53294148977005,"sku":"10625ES10","price":140.0,"currency_code":"USD","in_stock":true},{"title":"100 KU","offer_id":53294159200621,"sku":"10625ES60","price":1025.0,"currency_code":"USD","in_stock":true},{"title":"250 KU","offer_id":53294159233389,"sku":"10625ES72","price":2401.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/322e-167030612134953504.png?v=1778101062","url":"https:\/\/www.ebiohippo.com\/products\/t7-rna-polymerase-gmp-grade-250-u-mu-l-bhz20800044","provider":"BioHippo","version":"1.0","type":"link"}