{"product_id":"taf15-antibody-tata-binding-protein-associated-factor-2n-bha17135961","title":"TAF15 Antibody \/ TATA-binding protein-associated factor 2N","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTAF15 Antibody \/ TATA-binding protein-associated factor 2N is a anti-TAF15 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TAF15\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTAF15 antibody detects TATA-binding protein-associated factor 2N, an RNA-binding protein that participates in transcription initiation, RNA splicing, and stress granule dynamics. The UniProt recommended name is TATA-binding protein-associated factor 2N (TAF15). This multifunctional protein is a member of the FET family of RNA-binding proteins, which includes FUS and EWSR1, all of which link transcriptional and post-transcriptional gene regulation.\u003cbr\u003e\u003cbr\u003eFunctionally, TAF15 antibody identifies a 592-amino-acid nuclear protein characterized by RNA recognition motifs (RRMs), a low-complexity prion-like domain, and a glycine-rich C-terminus. TAF15 interacts with the TFIID complex, serving as a coactivator in transcription initiation by RNA polymerase II. It also binds nascent transcripts, coordinating RNA processing and export. Through its prion-like domain, TAF15 can assemble into reversible RNA-protein granules under stress, supporting mRNA storage and translational regulation.\u003cbr\u003e\u003cbr\u003eThe TAF15 gene is located on chromosome 17q11.2 and is ubiquitously expressed, with particularly high levels in neurons and other transcriptionally active cells. TAF15 acts at the interface of chromatin remodeling and RNA metabolism, modulating transcriptional output and transcript stability. It regulates genes involved in differentiation, cell cycle control, and stress response, serving as a key integrator of nuclear RNA metabolism.\u003cbr\u003e\u003cbr\u003eClinically, TAF15 has been implicated in oncogenic translocations and neurodegenerative disorders. Chromosomal rearrangements involving TAF15 generate fusion oncoproteins such as TAF15-NR4A3 and TAF15-CREB1, which act as aberrant transcriptional activators in sarcomas and leukemia. In the nervous system, abnormal aggregation of TAF15 has been linked to amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), where it contributes to RNA granule pathology and cytoplasmic mislocalization.\u003cbr\u003e\u003cbr\u003eTAF15 antibody is widely used in RNA biology, transcriptional regulation, and neurodegeneration research. It is suitable for immunoblotting, immunofluorescence, and chromatin immunoprecipitation to detect TAF15 localization and activity. This antibody supports studies of RNA processing, stress granule formation, and oncogenic fusions. In translational research, TAF15 detection aids in characterizing RNA-protein interactions and gene expression networks underlying disease.\u003cbr\u003e\u003cbr\u003eStructurally, TAF15 combines RNA-binding RRMs with intrinsically disordered regions that mediate multivalent interactions with RNA and chromatin-associated proteins.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TAF15 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TAF15 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TAF15 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TAF15 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TAF15+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310844269,"sku":"FY13059","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_81a906bf-a0f0-47d0-9c6b-80bdd37ce5f0.jpg?v=1782237070","url":"https:\/\/www.ebiohippo.com\/products\/taf15-antibody-tata-binding-protein-associated-factor-2n-bha17135961","provider":"BioHippo","version":"1.0","type":"link"}