{"product_id":"taf3-antibody-tata-box-binding-protein-associated-factor-3-bha17136224","title":"TAF3 Antibody \/ TATA-box binding protein associated factor 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTAF3 Antibody \/ TATA-box binding protein associated factor 3 is a anti-TAF3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30T12 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TAF3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30T12, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTAF3 antibody detects TATA-box binding protein associated factor 3, encoded by the TAF3 gene. TATA-box binding protein associated factor 3 is a subunit of the transcription factor IID complex, which is required for transcription initiation by RNA polymerase II. TAF3 antibody provides researchers with a valuable tool for studying gene regulation, transcription initiation, and chromatin dynamics.\u003cbr\u003e\u003cbr\u003eTATA-box binding protein associated factor 3 interacts directly with TATA-box binding protein and other TAF subunits to stabilize the transcription preinitiation complex at promoter regions. Research using TAF3 antibody has shown that it also recognizes histone modifications, particularly H3K4me3, through its plant homeodomain finger. This allows TAF3 to couple chromatin state with transcription initiation, providing a mechanism for epigenetic regulation of gene expression.\u003cbr\u003e\u003cbr\u003eStudies with TAF3 antibody have revealed that TATA-box binding protein associated factor 3 plays critical roles during development and differentiation. It is required for myogenic differentiation and early embryonic gene expression programs. Loss of TAF3 disrupts promoter recognition and impairs transcriptional activation, highlighting its central function in developmental biology.\u003cbr\u003e\u003cbr\u003eDysregulation of TATA-box binding protein associated factor 3 has been linked to disease. Research using TAF3 antibody has shown that altered expression contributes to cancer by modifying transcriptional programs. Because of its ability to read epigenetic marks, TAF3 is a key regulator of gene networks that govern proliferation and differentiation. These findings underscore its importance in both normal physiology and pathology.\u003cbr\u003e\u003cbr\u003eTAF3 antibody is widely applied in chromatin immunoprecipitation, western blotting, and immunohistochemistry. Chromatin immunoprecipitation maps TAF3 binding at promoters, western blotting detects full-length and isoform expression, and immunohistochemistry localizes TAF3 in nuclei across tissues. These applications make TAF3 antibody indispensable for transcription research.\u003cbr\u003e\u003cbr\u003eBy providing validated TAF3 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TAF3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TAF3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TAF3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TAF3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TAF3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047322607981,"sku":"FY13322","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1cda5e82-f400-403f-b039-1154165e3d35.jpg?v=1772019426","url":"https:\/\/www.ebiohippo.com\/products\/taf3-antibody-tata-box-binding-protein-associated-factor-3-bha17136224","provider":"BioHippo","version":"1.0","type":"link"}