{"product_id":"taf3-antibody-transcription-initiation-factor-tfiid-subunit-3-bha17136032","title":"TAF3 Antibody \/ Transcription initiation factor TFIID subunit 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTAF3 Antibody \/ Transcription initiation factor TFIID subunit 3 is a anti-TAF3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TAF3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTAF3 antibody detects Transcription initiation factor TFIID subunit 3, a component of the TFIID complex that regulates RNA polymerase II-dependent transcription. The UniProt recommended name is Transcription initiation factor TFIID subunit 3 (TAF3). This subunit serves as both a structural and regulatory component linking core promoter recognition to transcriptional activation.\u003cbr\u003e\u003cbr\u003eFunctionally, TAF3 antibody identifies a 929-amino-acid nuclear protein that contains a PHD finger domain recognizing histone H3K4me3, a chromatin mark associated with active transcription. TAF3 interacts with the TATA-binding protein (TBP) and other TAFs to form the pre-initiation complex, directing RNA polymerase II to target promoters. It also mediates enhancer-promoter looping during gene activation.\u003cbr\u003e\u003cbr\u003eThe TAF3 gene is located on chromosome 10q22.1 and is broadly expressed in embryonic and differentiated tissues. In muscle and hematopoietic cells, TAF3 cooperates with transcription factors like MyoD and C\/EBP to regulate lineage-specific gene expression. Through its histone mark recognition, TAF3 connects epigenetic signals to transcriptional initiation.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of TAF3 has been linked to developmental disorders and cancers. Altered TAF3 expression affects chromatin accessibility and transcriptional fidelity, contributing to tumor progression or differentiation defects. Research using TAF3 antibody supports studies in transcription regulation, epigenetics, and cell fate determination.\u003cbr\u003e\u003cbr\u003eTAF3 antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect transcriptional machinery components. \u003cbr\u003e\u003cbr\u003eStructurally, Transcription initiation factor TFIID subunit 3 contains a plant homeodomain (PHD) that binds methylated histones, enabling crosstalk between chromatin and transcriptional complexes. This antibody allows detailed analysis of TAF3's role in gene regulation and epigenetic control.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TAF3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TAF3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TAF3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TAF3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TAF3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312875885,"sku":"FY13130","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7c21527f-63cd-4746-8706-6595aed6c713.jpg?v=1772019402","url":"https:\/\/www.ebiohippo.com\/products\/taf3-antibody-transcription-initiation-factor-tfiid-subunit-3-bha17136032","provider":"BioHippo","version":"1.0","type":"link"}