{"product_id":"taf6-antibody-tata-box-binding-protein-associated-factor-6-bha17136162","title":"TAF6 Antibody \/ TATA-box binding protein-associated factor 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTAF6 Antibody \/ TATA-box binding protein-associated factor 6 is a anti-TAF6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TAF6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTAF6 antibody detects TATA-box binding protein-associated factor 6, a core subunit of the transcription factor IID (TFIID) complex that plays a central role in the initiation of RNA polymerase II-dependent transcription. The UniProt recommended name is Transcription initiation factor TFIID subunit 6 (TAF6). This protein is one of several TBP-associated factors (TAFs) that assemble with the TATA-binding protein (TBP) to recognize and bind core promoter regions, facilitating recruitment of the transcriptional machinery and gene activation.\u003cbr\u003e\u003cbr\u003eTAF6 is an integral structural and functional component of TFIID, contributing to promoter recognition, nucleosome remodeling, and transcription start site selection. The TFIID complex serves as a scaffold for preinitiation complex formation, interacting with transcriptional regulators, general transcription factors, and coactivators. TAF6 participates in DNA binding and mediates protein-protein interactions with other TAF subunits, including TAF9 and TAF12, forming submodules that stabilize TFIID assembly and promoter binding.\u003cbr\u003e\u003cbr\u003eThe TAF6 antibody identifies a protein of approximately 68 kDa encoded by the TAF6 gene on chromosome 7q22.3. Multiple isoforms of TAF6 are generated through alternative splicing, including the pro-apoptotic TAF6? variant, which lacks a short exon and promotes programmed cell death independently of p53 signaling. The balance between canonical TAF6 and TAF6? isoforms influences cellular responses to stress and apoptosis, highlighting the protein's dual role in both transcriptional regulation and cell survival pathways.\u003cbr\u003e\u003cbr\u003eFunctionally, TAF6 contributes to basal and regulated transcription by integrating signals from transcriptional activators and chromatin modifiers. It participates in the regulation of genes involved in cell cycle progression, DNA repair, and differentiation. TAF6 dysfunction or altered expression has been associated with impaired gene regulation and has been investigated in the context of cancer and neurodegenerative disorders, where transcriptional control is disrupted.\u003cbr\u003e\u003cbr\u003eTAF6 antibody is useful for applications such as immunoblotting, immunohistochemistry, and immunofluorescence to study transcription complex composition, promoter occupancy, and RNA polymerase II recruitment. It enables detection of TAF6 in nuclear extracts, cultured cells, and tissue samples, supporting research into transcriptional regulation and stress-induced gene expression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TAF6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TAF6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TAF6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TAF6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TAF6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321133421,"sku":"FY13260","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b15b1243-e8c3-452d-949e-a0dd9f131ee2.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/taf6-antibody-tata-box-binding-protein-associated-factor-6-bha17136162","provider":"BioHippo","version":"1.0","type":"link"}