{"product_id":"taq-dna-ligase-40-u-mu-l-bhz20800074","title":"Taq DNA Ligase (40 U\/µL)","description":"\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eTaq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl groups of two adjacent oligonucleotides hybridized to the same complementary target DNA strand. This catalytic reaction occurs only when the two oligonucleotides are perfectly paired with the complementary target DNA and there is no gap between the two oligonucleotides. Therefore, it can be used to detect single-base substitutions. Taq DNA Ligase uses NAD+ as a cofactor and is active in the temperature range of 45°C to 65°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eSpecific detection of alleles using ligase detection reaction and ligase chain reaction\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eMutagenesis by PCR amplification incorporating phosphorylated oligonucleotides\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan lang=\"EN-US\"\u003eHomologous recombination\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cdiv align=\"center\"\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"573\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"141\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eConcentration\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"431\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e40 U\/μL\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"141\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eSource\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"431\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eRecombinant E. coli strain containing the ligase gene cloned from Thermus aquaticus HB8.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"141\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eUnit Definition\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"431\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eOne unit of activity is defined as the amount of enzyme required to ligate 50% of 1 µg of λDNA fragments digested with BstEII (12 bp sticky ends) within 15 minutes at 45°C in a 50 µL reaction volume\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"141\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eInactivation conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"431\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eIncubate at 45°C for 15 minutes. Add stop dye (50% glycerol, 50 mM EDTA, and bromophenol blue) to terminate the reaction\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"102%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"21%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eComponents No.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"28%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eName\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e11051ES80\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e11051ES84\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e11051ES92\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e11051-A\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"28%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"SV\"\u003eTaq DNA Ligase (40 U\/µL)\u003c\/span\u003e\u003cspan lang=\"SV\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003ca name=\"OLE_LINK10\"\u003e\u003c\/a\u003e\u003ca name=\"OLE_LINK11\"\u003e\u003c\/a\u003e\u003ca name=\"OLE_LINK12\"\u003e\u003c\/a\u003e\u003cspan lang=\"EN-US\"\u003e25 μL\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e50 μL\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e5×50 μL\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"21%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e11051-B\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"28%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"SV\"\u003e10×Taq DNA Ligase Buffer\u003c\/span\u003e（\u003cspan lang=\"SV\"\u003eNAD+\u003c\/span\u003e）\u003cspan lang=\"SV\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e250 μL\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e500 μL\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"16%\"\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003e5×500 μL\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp align=\"left\"\u003e\u003cspan lang=\"EN-US\"\u003eThis product should be stored at -\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e25 ~ -15\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e℃\u003c\/span\u003e\u003cspan lang=\"EN-US\"\u003e for 1 year.\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1000 U","offer_id":53294150943085,"sku":"11051ES80","price":55.0,"currency_code":"USD","in_stock":true},{"title":"2000 U","offer_id":53294156677485,"sku":"11051ES84","price":110.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294156710253,"sku":"11051ES92","price":435.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20190508155908726_2fca3565-765c-4681-878b-a88b12d18cc1.webp?v=1778110764","url":"https:\/\/www.ebiohippo.com\/products\/taq-dna-ligase-40-u-mu-l-bhz20800074","provider":"BioHippo","version":"1.0","type":"link"}