{"product_id":"tbr1-antibody-t-box-brain-protein-1-bha17136005","title":"TBR1 Antibody \/ T-box brain protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTBR1 Antibody \/ T-box brain protein 1 is a anti-TBR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TBR1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTBR1 antibody detects T-box brain protein 1, a neuron-specific transcription factor that regulates cortical development and neuronal differentiation. The UniProt recommended name is T-box brain protein 1 (TBR1). This DNA-binding transcription factor belongs to the T-box family and is essential for neocortical layer specification, axonal guidance, and synaptic connectivity.\u003cbr\u003e\u003cbr\u003eFunctionally, TBR1 antibody identifies a 682-amino-acid nuclear protein that binds T-box DNA consensus sequences to regulate the expression of genes involved in neuronal subtype identity and projection targeting. TBR1 interacts with transcriptional co-regulators such as CASK, FOXP2, and SOX5, forming complexes that guide early cortical neuron differentiation and maturation.\u003cbr\u003e\u003cbr\u003eThe TBR1 gene is located on chromosome 2q24.2 and is expressed predominantly in postmitotic glutamatergic neurons of the developing and adult cerebral cortex. It functions as a key determinant of cortical layer VI identity and regulates axonal projections to subcortical targets. TBR1 is a downstream effector of the transcription factor PAX6, linking progenitor differentiation to neuronal specialization.\u003cbr\u003e\u003cbr\u003ePathologically, TBR1 mutations have been associated with autism spectrum disorders, intellectual disability, and cortical malformations. Its dysregulation disrupts neuronal migration, synapse formation, and interhemispheric connectivity. Research using TBR1 antibody provides insights into neurodevelopmental biology, transcriptional regulation, and disease mechanisms involving cortical circuitry.\u003cbr\u003e\u003cbr\u003eTBR1 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry to label developing cortical neurons and nuclear transcription factors. \u003cbr\u003e\u003cbr\u003eStructurally, T-box brain protein 1 contains a central T-box DNA-binding domain that mediates sequence-specific gene regulation. It acts as both an activator and repressor depending on its interacting partners. This antibody enables researchers to explore TBR1's roles in cortical specification and neural differentiation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TBR1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TBR1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TBR1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TBR1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TBR1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312908653,"sku":"FY13103","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8b527986-6ad4-4323-9e0f-cf53fbb11f4c.jpg?v=1782237090","url":"https:\/\/www.ebiohippo.com\/products\/tbr1-antibody-t-box-brain-protein-1-bha17136005","provider":"BioHippo","version":"1.0","type":"link"}