{"product_id":"tcf-lef-stembright-luciferase-ips-cell-pool-wnt-pathway-bhc18200144","title":"TCF\/LEF StemBright™ Luciferase iPS Cell Pool (Wnt Pathway)","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table tr:nth-child(even) th{background:#dce8fb}\n.bhp-desc .bhp-spec-table tr:nth-child(even) td{background:#f0f6ff}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-bottom:10px}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444;margin:10px 0 14px}\n.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eThe Wnt signaling pathway is a well described regulator of organism development and cell fate specification. Cellular pluripotency and differentiation of multiple lineages, including cardiomyocytes and neurons are modulated by this pathway. There are 19 Wnt ligands and 10 Frizzled receptors, which stimulate canonical as well as non-canonical Wnt pathways. In the canonical pathway, cell activation by a Wnt ligand leads to the stabilization and nuclear translocation of β-catenin and subsequent activation of TCF\/LEF transcription factor. The Wnt signaling pathway has recently been shown to play a role in cell cycle reentry and proliferation of iPS-derived cardiomyocytes. It is a therapeutic target in the treatment of neuropsychiatric disorders.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eCell Signaling\u003c\/span\u003e\n\u003cp\u003eThe TCF\/LEF (T-cell factor\/lymphoid enhancing factor) StemBright™ Luciferase Cells contain a firefly luciferase gene under the control of TCF\/LEF responsive elements, stably integrated into induced Pluripotent Stem (iPS) cells. TCF\/LEF transcription factors are downstream of the Wnt signaling pathway. This cell pool is validated for its response to GSK3β inhibitor CHIR-99021, which activates the Wnt signaling pathway in human pluripotent stem cells.\u003c\/p\u003e\n\u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eNon-disease human ips cell line (ixcells 30hu-002)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Method\u003c\/th\u003e\n\u003ctd\u003eLentivirus (#79787)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSupplied As\u003c\/th\u003e\n\u003ctd\u003eEach vial contains 2 x 10\u003csup\u003e6\u003c\/sup\u003e cells in 1 ml of iPS cell freezing medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHarmonized Tariff Code\u003c\/th\u003e\n\u003ctd\u003e3002-5900\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eQuality Control \u0026amp; Validation\u003c\/h2\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003e✓ Mycoplasma-Tested\u003c\/span\u003e\n\u003cp\u003eThe cell line has been screened to confirm the absence of Mycoplasma species.\u003c\/p\u003e\n\u003ch2\u003eRegulatory Information\u003c\/h2\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLicense Required\u003c\/strong\u003eYes (IPS, Academia Japan)\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLiving Modified Organism\u003c\/strong\u003eYes\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003eLicense Disclosure\u003c\/h2\u003e\n\u003cdiv class=\"bhp-license-box\"\u003eThe iPSC technology is protected by several patents, including US patent Nos. 8048999, 8058065, 8129187, 8278104, 8530238, 8900871, 9404124, 9499797, 10519425, and patent pending, for which iPS Academia Japan, Inc. has been granted license rights with a sub-licensable right. The purchase of this cell line grants you a 10-year license to use this cell line in your immediate laboratory for internal research purposes. Commercial use of this cell line is not allowed. Commercial use requires the appropriate license from iPS Academia Japan, Inc. This cell line is for research use only, not for therapeutic or prophylactic use in humans or animals. Use in humans is strictly prohibited. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of the cell line in any way. Modification of this cell line or transfer to another facility of the cells requires a separate license or additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use and does not accept any liability in connection with the handling or use of the cell line. Visit bpsbioscience.com\/license for the label license and other key information about this product. Troubleshooting Guide : Visit bpsbioscience.com\/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eRelated Products: Cat. #60501, 27780-1, 79787\u003c\/p\u003e\n\u003cp\u003eRequired Accessories: Cat. #79796\u003c\/p\u003e\n\u003c\/div\u003e","brand":"BPS Bioscience","offers":[{"title":"1 Vial","offer_id":53322317824365,"sku":"78515","price":5655.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/78515_1.png?v=1778636459","url":"https:\/\/www.ebiohippo.com\/products\/tcf-lef-stembright-luciferase-ips-cell-pool-wnt-pathway-bhc18200144","provider":"BioHippo","version":"1.0","type":"link"}