{"product_id":"tcirg1-antibody-bha10535386","title":"TCIRG1 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-TCIRG1 antibody raised in Rabbit, with confirmed utility in ELISA, IHC, IF. It is designed to detect TCIRG1 protein in Human and supports researchers working in signal transduction contexts where consistent antibody performance is required.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human V-type proton ATPase 116 kDa subunit a isoform 3 protein (76-182AA) — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Compatible with standard Protein A\/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eTCIRG1\u003c\/strong\u003e (also referred to as a3, Atp 6i, Atp6i, ATP6N1C, ATP6V0A3, ATPase H+ transporting 116kD, OC 116, OC 116 kDa, OC 116kDa, OC-116 kDa, OC116, OPTB 1, OPTB1, Osteoclastic proton pump 116 kDa subunit, Specific 116 kDa vacuolar proton pump subunit, Stv 1, Stv1, T cell immune regulator 1, T cell immune regulator 1 ATPase H+ transporting lysosomal V0 subunit A, T cell immune regulator 1 ATPase H+ transporting lysosomal V0 subunit A3, T cell immune response cDNA 7, T cell immune response cDNA7 protein, T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a, T-cell immune regulator 1, T-cell immune response cDNA7 protein, TCIRG 1, TCIRG1, TIRC 7, TIRC7, V ATPase 116 kDa, V ATPase 116 kDa isoform a3, V type proton ATPase 116 kDa subunit a, V-ATPase 116 kDa isoform a3, V-type proton ATPase 116 kDa subunit a isoform 3, Vacuolar proton translocating ATPase 116 kDa subunit A, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3, Vph 1, Vph1, VPP3_HUMAN) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of TCIRG1 biology, including known isoforms, interactors, and disease-relevant expression patterns in signal transduction.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Sandwich or indirect ELISA can quantify soluble target in biological fluids or culture supernatants. Ensure samples fall within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e IHC visualizes target in FFPE or frozen tissue sections. Optimize antigen retrieval method and secondary detection for each tissue type.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIF:\u003c\/strong\u003e Immunofluorescence enables subcellular localization in fixed cells or tissue sections. Fixation and permeabilization conditions are critical for epitope accessibility.\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio datasheet: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53241534611821,"sku":"CSB-PA615690LA01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53241757991277,"sku":"CSB-PA615690LA01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA615690LA01HU-IHC-1.jpg?v=1776697531","url":"https:\/\/www.ebiohippo.com\/products\/tcirg1-antibody-bha10535386","provider":"BioHippo","version":"1.0","type":"link"}