{"product_id":"tcirg1-antibody-t-cell-immune-regulator-1-bha17135494","title":"TCIRG1 Antibody \/ T-cell immune regulator 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTCIRG1 Antibody \/ T-cell immune regulator 1 is a anti-TCIRG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TCIRG1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTCIRG1 antibody detects T-cell immune regulator 1, a subunit of the vacuolar-type H+-ATPase (V-ATPase) proton pump that acidifies intracellular compartments and bone resorption lacunae. TCIRG1 is essential for osteoclast function, lysosomal acidification, and immune regulation. The TCIRG1 antibody is widely used in osteoclast biology, immunology, and cell physiology research to study proton transport, lysosomal function, and bone metabolism.\u003cbr\u003e\u003cbr\u003eTCIRG1 is encoded by the TCIRG1 gene on human chromosome 11q13.2. The protein is approximately 830 amino acids long and represents the a3 isoform of the V-ATPase a subunit. It localizes to the ruffled border membrane of osteoclasts, where it pumps protons into the bone resorption lacuna, dissolving mineralized matrix and enabling bone remodeling. In other cell types, TCIRG1 contributes to lysosomal acidification and vesicle trafficking.\u003cbr\u003e\u003cbr\u003eThe TCIRG1 antibody detects a 100 kilodalton band by western blot and shows membrane and vesicular staining under immunofluorescence microscopy. Mutations in TCIRG1 cause autosomal recessive osteopetrosis, a disorder characterized by defective bone resorption, increased bone density, and bone marrow failure. In immune cells, TCIRG1 expression regulates antigen processing and presentation by acidifying endolysosomal compartments.\u003cbr\u003e\u003cbr\u003eBeyond bone biology, TCIRG1 plays roles in metabolic adaptation and intracellular pH regulation. It interacts with other V-ATPase subunits to control organelle acidification in neurons, hepatocytes, and epithelial cells. Dysregulation of TCIRG1 or V-ATPase activity contributes to cancer cell invasion and multidrug resistance due to altered extracellular acidification.\u003cbr\u003e\u003cbr\u003eAs a vital component of the proton pump machinery, TCIRG1 links acid-base regulation with immune and skeletal homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TCIRG1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TCIRG1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TCIRG1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TCIRG1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TCIRG1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294361965,"sku":"FY12591","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_57b6999c-90a5-4b0a-9d5c-0e6691695596.jpg?v=1782237029","url":"https:\/\/www.ebiohippo.com\/products\/tcirg1-antibody-t-cell-immune-regulator-1-bha17135494","provider":"BioHippo","version":"1.0","type":"link"}