{"product_id":"tcp1-gamma-antibody-cct3-bha17103248","title":"TCP1 gamma Antibody \/ CCT3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eTCP1 gamma antibody supplied as a purified reagent for FACS, IHC-P, WB in Human samples. This product is a polyclonal (rabbit origin) antibody (host: Rabbit; isotype: Rabbit Ig) intended for research use only. The target is commonly annotated with cytoplasmic localization context, which may inform staining patterns.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); host Rabbit; isotype Rabbit Ig.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Purified.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e FACS, IHC-P, WB.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A portion of amino acids 298-326 from the human protein was used as the immunogen for the TCP1 gamma antibody..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Cytoplasmic (annotation-level guidance; cell state and isoforms can shift patterns).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eTCP1 gamma is the intended antigen for this primary antibody. Reported biological context includes: CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Subcellular localization information (Cytoplasmic) can be useful when interpreting IF\/ICC patterns and selecting compartment-enriched lysates for WB.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e   \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eFACS: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e   \u003cli\u003eIHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a polyclonal antibody, recognition spans multiple epitopes, which can improve detection across conformations but may broaden background depending on sample context.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProtKB entry (P49368) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P49368\/entry - NCBI Gene search (TCP1 gamma) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TCP1+gamma - Ensembl search (TCP1 gamma) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TCP1+gamma - PubMed search (TCP1 gamma) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TCP1+gamma - Reactome pathway search (TCP1 gamma) — Reactome — https:\/\/reactome.org\/content\/query?q=TCP1+gamma --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.08 ml","offer_id":53043232702829,"sku":"F54786-0.08ML","price":205.0,"currency_code":"USD","in_stock":true},{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.4 ml","offer_id":53043648233837,"sku":"F54786-0.4ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b99cb4f8-c27f-413b-a780-cb925c357350.jpg?v=1771934286","url":"https:\/\/www.ebiohippo.com\/products\/tcp1-gamma-antibody-cct3-bha17103248","provider":"BioHippo","version":"1.0","type":"link"}