{"product_id":"tctn1-antibody-tectonic-1-bha17136083","title":"TCTN1 Antibody \/ Tectonic 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTCTN1 Antibody \/ Tectonic 1 is a anti-TCTN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Actin filaments, Primary cilium, Nucleoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TCTN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTCTN1 antibody detects Tectonic-1, a membrane-associated protein essential for ciliogenesis and Hedgehog signaling. The UniProt recommended name is Tectonic-1 (TCTN1). This protein localizes to the ciliary transition zone, where it participates in the formation and maintenance of the primary cilium, a sensory organelle crucial for signal transduction during embryonic development and tissue patterning.\u003cbr\u003e\u003cbr\u003eFunctionally, TCTN1 antibody identifies a 765-amino-acid protein that interacts with other tectonic complex components such as TCTN2, TCTN3, and MKS1. TCTN1 regulates protein trafficking into the cilium and ensures the proper localization of signaling receptors, including those of the Hedgehog pathway. It is indispensable for normal morphogenesis and neural tube patterning.\u003cbr\u003e\u003cbr\u003eThe TCTN1 gene is located on chromosome 12q24.11 and is expressed in ciliated cells throughout various tissues, including brain, kidney, and retina. Its activity maintains ciliary structure and mediates signal coordination during development and organogenesis.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in TCTN1 lead to ciliopathies such as Joubert and Meckel-Gruber syndromes, which are characterized by developmental defects in the brain, kidneys, and eyes. Dysfunction of TCTN1 disrupts ciliary signaling, resulting in structural malformations and impaired embryonic patterning. Research using TCTN1 antibody supports studies in ciliary biology, developmental signaling, and ciliopathy mechanisms.\u003cbr\u003e\u003cbr\u003eTCTN1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ciliary transition zone proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Tectonic-1 contains multiple coiled-coil regions and a C-terminal transmembrane domain that anchor it to the ciliary membrane. This antibody enables investigation of TCTN1's function in ciliogenesis and Hedgehog pathway regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TCTN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TCTN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TCTN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TCTN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TCTN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047317954925,"sku":"FY13181","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_34ab06e4-e5b6-4fe5-a436-b6b86fcd6399.jpg?v=1782237079","url":"https:\/\/www.ebiohippo.com\/products\/tctn1-antibody-tectonic-1-bha17136083","provider":"BioHippo","version":"1.0","type":"link"}