{"product_id":"tdp1-antibody-tyrosyl-dna-phosphodiesterase-1-bha17136173","title":"TDP1 Antibody \/ Tyrosyl-DNA phosphodiesterase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTDP1 Antibody \/ Tyrosyl-DNA phosphodiesterase 1 is a anti-TDP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TDP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTDP1 antibody detects Tyrosyl-DNA phosphodiesterase 1, a DNA repair enzyme that removes covalently trapped topoisomerase I-DNA complexes and repairs DNA single-strand breaks. The UniProt recommended name is Tyrosyl-DNA phosphodiesterase 1 (TDP1). This enzyme maintains genome stability by resolving abortive topoisomerase I intermediates generated during DNA replication and transcription.\u003cbr\u003e\u003cbr\u003eFunctionally, TDP1 antibody identifies a 608-amino-acid nuclear enzyme that hydrolyzes the phosphodiester bond linking tyrosine residues of topoisomerase I to the 3? end of DNA. TDP1 also processes other blocked DNA termini generated by oxidative damage or chemotherapeutic agents. Its catalytic mechanism involves two histidine residues acting in a transesterification reaction, forming a transient covalent intermediate. TDP1 cooperates with PARP1 and XRCC1 in the single-strand break repair pathway to restore DNA integrity.\u003cbr\u003e\u003cbr\u003eThe TDP1 gene is located on chromosome 14q32.11 and is expressed in a broad range of tissues, with highest levels in brain, thymus, and testis. Expression increases in response to DNA damage and genotoxic stress, indicating a role in stress-induced DNA repair. TDP1 activity supports genomic maintenance in neurons, where oxidative DNA damage is frequent.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in TDP1 cause spinocerebellar ataxia with axonal neuropathy (SCAN1), a rare neurodegenerative disorder characterized by progressive motor impairment and neuronal DNA repair deficiency. In cancer, TDP1 confers resistance to topoisomerase I inhibitors such as camptothecin and topotecan by removing drug-stabilized cleavage complexes. Research using TDP1 antibody supports studies in DNA repair, cancer pharmacology, and neurodegeneration.\u003cbr\u003e\u003cbr\u003eTDP1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect DNA repair enzymes. \u003cbr\u003e\u003cbr\u003eStructurally, Tyrosyl-DNA phosphodiesterase 1 consists of two domains forming an alpha\/beta sandwich fold that positions catalytic histidine residues in the active site. The enzyme's flexible loops accommodate diverse DNA substrates, and phosphorylation or SUMOylation modulates its repair activity. This antibody facilitates investigation of TDP1's role in DNA repair, topoisomerase regulation, and neuroprotection.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TDP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TDP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TDP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TDP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TDP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321002349,"sku":"FY13271","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_affdc18e-d717-4efe-ae41-89b194d19593.jpg?v=1782237095","url":"https:\/\/www.ebiohippo.com\/products\/tdp1-antibody-tyrosyl-dna-phosphodiesterase-1-bha17136173","provider":"BioHippo","version":"1.0","type":"link"}