{"product_id":"terf2ip-antibody-bha17135472","title":"TERF2IP Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTERF2IP Antibody is a anti-TERF2IP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TERF2IP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTERF2IP antibody detects Telomeric repeat-binding factor 2-interacting protein, a critical component of the shelterin complex that protects chromosome ends and maintains telomere stability. Also known as RAP1, TERF2IP interacts with TERF2 to regulate telomeric structure, repress DNA damage signaling, and prevent end-to-end chromosome fusions. The TERF2IP antibody is widely used in studies of chromosome biology, DNA repair, and aging to investigate telomere function and genome protection mechanisms.\u003cbr\u003e\u003cbr\u003eTERF2IP is encoded by the TERF2IP gene located on human chromosome 16p13.3. The protein is approximately 399 amino acids long and consists of an N-terminal BRCT domain involved in protein interactions and a C-terminal Myb-like DNA-binding domain that anchors it to telomeric DNA via TERF2. TERF2IP localizes to telomeres throughout the cell cycle, forming a stabilizing subcomplex with TERF2 that suppresses activation of the ATM kinase pathway, a major mediator of DNA damage response.\u003cbr\u003e\u003cbr\u003eThe TERF2IP antibody detects a 45-60 kilodalton band by western blot and shows punctate nuclear foci corresponding to telomere clusters by immunofluorescence. Beyond its telomeric role, TERF2IP functions in transcriptional regulation through the NF-kappaB pathway. In the cytoplasm, it interacts with the IKK complex and influences inflammatory signaling, demonstrating dual nuclear and cytoplasmic roles.\u003cbr\u003e\u003cbr\u003eLoss of TERF2IP function results in telomere uncapping, increased DNA damage foci, and cellular senescence. Mutations or mislocalization of TERF2IP have been linked to premature aging syndromes, cancer progression, and metabolic disorders. Its overexpression enhances survival of tumor cells under stress by repressing telomeric DNA damage checkpoints and promoting NF-kappaB-mediated gene expression.\u003cbr\u003e\u003cbr\u003eBecause TERF2IP integrates telomere maintenance with stress and inflammatory signaling, it serves as an important marker for genomic stability and cancer biology research.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TERF2IP - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TERF2IP - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TERF2IP - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TERF2IP - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TERF2IP+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292985709,"sku":"FY12569","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_03773bca-63a9-425a-9849-85d6d6203234.jpg?v=1782237019","url":"https:\/\/www.ebiohippo.com\/products\/terf2ip-antibody-bha17135472","provider":"BioHippo","version":"1.0","type":"link"}