{"product_id":"terminal-transferase-tdt-bhp21409884","title":"Terminal Transferase (TdT)","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eTarget identity:\u003c\/strong\u003e\u003cstrong\u003eTDT\u003c\/strong\u003e is a enzyme. It is typically cytosolic, nuclear, or organelle-localized (depends on isoform).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTDT\u003c\/strong\u003e is provided as a recombinant protein reagent for \u003cstrong\u003eresearch use only\u003c\/strong\u003e. Recombinant proteins are commonly used as defined molecular inputs in biochemical and cell-free systems, enabling controlled interrogation of binding, activity, and pathway-relevant interactions.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProtein identity context:\u003c\/strong\u003e TDT (expression region Terminal Transferase (TdT) is expressed in E. coli.; approx. molecular weight 45 kDa).\u003c\/p\u003e\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eTDT\u003c\/strong\u003e supports biochemical transformations that can be read out as changes in substrate\/product balance or signaling intermediates. Recombinant enzymes enable controlled reconstitution experiments and mechanistic interrogation with defined inputs. This target is frequently explored in \u003cstrong\u003eMetabolism \u0026amp; Enzymology\u003c\/strong\u003e research contexts.\u003c\/p\u003e\u003ch2\u003eMolecular characteristics\u003c\/h2\u003e\u003cp\u003eKey molecular attributes can influence binding behavior, stability, and assay background—especially for multimeric, disulfide-rich, or PTM-dependent targets.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression region:\u003c\/strong\u003e Terminal Transferase (TdT) is expressed in E. coli.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 45 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;90%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e 100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003ePost-translational considerations:\u003c\/strong\u003e Expression host can influence folding and PTMs (e.g., glycosylation, disulfide bonds), which may impact stability or binding depending on protein class. For enzymes, cofactor binding state and oligomerization can also shape apparent activity in reconstituted assays.\u003c\/p\u003e\u003ch2\u003eStructural and biochemical features\u003c\/h2\u003e\u003cp\u003eEnzyme behavior can be influenced by oligomerization, cofactor state, and local microenvironment, which may change apparent kinetics in simplified assay formats.\u003c\/p\u003e\u003ch2\u003eExpression and purification strategy\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003ePurification:\u003c\/strong\u003e Affinity-chromatography. Purification approach and formulation influence sample homogeneity and background signal in downstream biochemical measurements.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eEndotoxin consideration:\u003c\/strong\u003e Reported endotoxin level is Please contact with the lab for this information.; this parameter can matter when recombinant proteins are used in cell-based systems sensitive to innate immune activation.\u003c\/p\u003e\u003ch2\u003eResearch interpretation\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch interpretation:\u003c\/strong\u003e Enzymatic readouts can reflect both abundance and catalytic state. Orthogonal measurements (substrate levels, product formation, and pathway markers) help clarify whether changes arise from expression, inhibition, or cofactor availability.\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 ug","offer_id":53001948627309,"sku":"BY380012-100UG","price":311.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53001948660077,"sku":"BY380012-1MG","price":1627.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/terminal-transferase-tdt-bhp21409884","provider":"BioHippo","version":"1.0","type":"link"}