{"product_id":"tev-protease-bhz10900155","title":"TEV Protease","description":"\u003cp\u003e\u003cstrong\u003eabm’s TEV Protease\u003c\/strong\u003e is an enhanced version of the site-specific protease derived from \u003cem\u003eTobacco Etch Virus\u003c\/em\u003e (TEV). This optimized enzyme offers superior \u003cstrong\u003eactivity\u003c\/strong\u003e, \u003cstrong\u003estability\u003c\/strong\u003e, and \u003cstrong\u003esite-specificity\u003c\/strong\u003e compared to the native form. TEV Protease cleaves fusion proteins with high precision at the \u003cstrong\u003eGln-Gly\u003c\/strong\u003e or \u003cstrong\u003eGln-Ser\u003c\/strong\u003e bond within the seven-amino acid recognition sequence \u003cstrong\u003eGlu-Asn-Leu-Tyr-Phe-Gln-Gly\/Ser\u003c\/strong\u003e (ENLYFQ(G\/S)).\u003c\/p\u003e\n\u003cp\u003eThe enzyme is highly effective across a broad range of temperatures (\u003cstrong\u003e4–30°C\u003c\/strong\u003e, with an optimum at \u003cstrong\u003e30°C\u003c\/strong\u003e) and pH values (\u003cstrong\u003e5.5–9.0\u003c\/strong\u003e), enabling flexible experimental conditions. Under optimal conditions, up to \u003cstrong\u003e99% cleavage\u003c\/strong\u003e can be achieved in just \u003cstrong\u003e1–2 hours\u003c\/strong\u003e. Additionally, the inclusion of a \u003cstrong\u003e6X-His tag\u003c\/strong\u003e at the N-terminus allows for convenient removal of TEV Protease post-cleavage using \u003cstrong\u003eNi-IDA Agarose Beads\u003c\/strong\u003e (\u003ca\u003eCat. No. G250\u003c\/a\u003e) via affinity chromatography.\u003c\/p\u003e\n\u003ctable class=\"bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\" style=\"margin: 30px 0;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eProduct Component\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd\u003eTEV Protease (10 U\/µl)\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e20X TEV Protease Reaction Buffer\u003c\/td\u003e\n\u003ctd\u003e1.0 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 mM DTT\u003c\/td\u003e\n\u003ctd\u003e500 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\u003ctable class=\"bhc-specs-table\" style=\"width:100%;border-collapse:collapse\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#1a5c58;color:#fff\"\u003e\n\u003cth style=\"padding:8px 12px;text-align:left\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"padding:8px 12px;text-align:left\"\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eEnzyme Type\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eProtease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eEnzyme Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"100 ul","offer_id":53294164771181,"sku":"E027","price":49.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/tev-protease-corrected.png?v=1778482143","url":"https:\/\/www.ebiohippo.com\/products\/tev-protease-bhz10900155","provider":"BioHippo","version":"1.0","type":"link"}