{"product_id":"tfap4-antibody-transcription-factor-ap-4-bha17135952","title":"TFAP4 Antibody \/ Transcription factor AP-4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eTFAP4 Antibody \/ Transcription factor AP-4 is a anti-TFAP4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TFAP4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eTFAP4 antibody detects Transcription factor AP-4, a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor that regulates cell proliferation, differentiation, and apoptosis. The UniProt recommended name is Transcription factor AP-4 (TFAP4). This protein binds to E-box DNA motifs (CAGCTG) to modulate gene expression involved in developmental signaling and oncogenic transformation.\u003cbr\u003e\u003cbr\u003eFunctionally, TFAP4 antibody identifies a 338-amino-acid nuclear transcription factor that acts as both an activator and repressor of transcription. TFAP4 dimerizes through its leucine zipper domain and binds to specific promoter sequences, influencing the expression of genes controlling cell cycle progression, epithelial-to-mesenchymal transition (EMT), and differentiation. It regulates genes such as MYC, CDKN1A (p21), and CCND1, linking growth control to transcriptional responses.\u003cbr\u003e\u003cbr\u003eThe TFAP4 gene is located on chromosome 16p13.3 and is broadly expressed in epithelial and immune cells. TFAP4 functions downstream of MYC as a key mediator of its transcriptional program. In oncogenesis, TFAP4 promotes proliferation, invasion, and metastasis by activating EMT-related transcription factors such as SNAIL and repressing cell adhesion molecules like E-cadherin. Conversely, under stress conditions, TFAP4 can trigger apoptotic pathways through p53-dependent transcriptional activation.\u003cbr\u003e\u003cbr\u003eTFAP4 also participates in development by regulating neuronal and epithelial differentiation. Its activity is modulated by post-translational modifications including phosphorylation and acetylation, which influence its DNA-binding affinity and cofactor recruitment. Dysregulation of TFAP4 expression has been linked to various cancers, including colorectal, breast, and gastric cancer, where it drives metastasis and stem-like phenotypes.\u003cbr\u003e\u003cbr\u003eTFAP4 antibody is widely used in transcriptional regulation, cancer biology, and developmental studies. It is suitable for western blotting, immunohistochemistry, and chromatin immunoprecipitation to detect TFAP4 protein levels and promoter occupancy. This antibody supports investigations into MYC signaling, cell cycle regulation, and transcriptional reprogramming. In oncology, TFAP4 detection provides insights into tumor progression and epithelial plasticity.\u003cbr\u003e\u003cbr\u003eStructurally, TFAP4 contains a bHLH-LZ domain for DNA binding and dimerization, along with transactivation and repression regions that recruit chromatin-modifying complexes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TFAP4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TFAP4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TFAP4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TFAP4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TFAP4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310614893,"sku":"FY13050","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_dbaf6eef-252c-4c49-b062-7ebb64404637.jpg?v=1782237074","url":"https:\/\/www.ebiohippo.com\/products\/tfap4-antibody-transcription-factor-ap-4-bha17135952","provider":"BioHippo","version":"1.0","type":"link"}